Isolation and Characterization of a Glycosylation Mutant from Schizosaccharomyces pombe

N-Linked oligosaccharides were elongated by glycosylation with mannose and galactose residues in the secretory pathway of Schizosaccharomyces pombe. The wild-type S. pombe cells were agglutinated by the additions of not only concanavalin A lectin, which is specific for mannose residues, but also PNA...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1996, Vol.60 (7), p.1156-1159
Main Authors: Kaoru, Takegawa, Naotaka, Tanaka, Mitsuaki, Tabuchi, Shojiro, Iwahara
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Biological and medical sciences
Biotechnology
Classical genetics, quantitative genetics, hybrids
Concanavalin A - chemistry
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
glactomannan
glycosylation
Glycosylation - drug effects
Hygromycin B - pharmacology
Lectins
Magnetic Resonance Spectroscopy
Mutation - physiology
Nlinked oligosaccharide
Oligosaccharides - genetics
Oligosaccharides - metabolism
Phenotype
Schizosaccharomyces - drug effects
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Thallophyta, bryophyta
Vanadates - pharmacology
Vegetals
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Summary:N-Linked oligosaccharides were elongated by glycosylation with mannose and galactose residues in the secretory pathway of Schizosaccharomyces pombe. The wild-type S. pombe cells were agglutinated by the additions of not only concanavalin A lectin, which is specific for mannose residues, but also PNA (from Arachis hypogaea) and RCA (Ricinus communis) lectins, which are specific for terminal galactose residues. By PNA-binding selection, we isolated an S. pombe mutant defective in protein glycosylation. The mutant cells, named gmsl, were not agglutinated by PNA or RCA. In contrast, agglutination of the gmsl cells by the addition of concanavalin A was markedly increased. Structural studies on N-linked oligosaccharides from gmsl mutant cells showed that the number of x-l,2-linked galactose residues wes markedly reduced, and unsubstituted x-l,6-linked polymannose outer chains were attached to the core oligosaccharides.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60.1156