A human canalicular multispecific organic anion transporter (cMOAT) gene is overexpressed in cisplatin-resistant human cancer cell lines with decreased drug accumulation

By targeting the ATP binding conserved domain in three ATP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance protein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new ATP binding cassette superfamily that was specifically enhanced in a cisplatin-r...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1996-09, Vol.56 (18), p.4124-4129
Main Authors: TANIGUCHI, K, WADA, M, KOHNO, K, NAKAMURA, T, KAWABE, T, KAWAKAMI, M, KAGOTANI, K, OKUMURA, K, AKIYAMA, S.-I, KUWANO, M
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Animals
Anion Transport Proteins
Antineoplastic agents
Base Sequence
Binding Sites
Biological and medical sciences
Carrier Proteins - biosynthesis
Carrier Proteins - genetics
Chromosome Mapping
Chromosomes, Human, Pair 10
Cisplatin - toxicity
Conserved Sequence
DNA Primers
DNA, Complementary
Drug Resistance, Neoplasm
Gene Expression
General aspects
Humans
In Situ Hybridization, Fluorescence
KB Cells
Male
Medical sciences
Molecular Sequence Data
Pharmacology. Drug treatments
Prostatic Neoplasms
Rats
Sequence Homology, Amino Acid
Urinary Bladder Neoplasms
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Summary:By targeting the ATP binding conserved domain in three ATP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance protein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new ATP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat canalicular multispecific organic anion transporter (cMOAT) was found and designated human cMOAT. Fluorescence in situ hybridization demonstrated the chromosomal locus of the gene on chromosome 10q24. The human cMOAT cDNA hybridized a 6.5-kb mRNA that was expressed 4- to 6-fold higher by three cisplatin-resistant cell lines derived from various human tumors exhibiting decreased drug accumulation. Human cMOAT may function as a cellular cisplatin transporter.
ISSN:0008-5472
1538-7445