Translocation of PKN from the Cytosol to the Nucleus Induced by Stresses

Effects of environmental stresses on the subcellular localization of PKN were investigated in NIH 3T3, BALB/c 3T3, and Rat-1 cells. The immunofluorescence of PKN resided prominently in the cytoplasmic region in nonstressed cells. When these cells were treated at 42 degrees C, there was a time-depend...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1996-09, Vol.93 (19), p.10195-10199
Main Authors: Mukai, Hideyuki, Miyahara, Masako, Sunakawa, Hiroko, Shibata, Hideki, Toshimori, Masanao, Kitagawa, Michinori, Shimakawa, Masaki, Takanaga, Hiromi, Ono, Yoshitaka
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Arsenites
Arsenites - pharmacology
BALB 3T3 cells
Cell Fractionation
Cell Line
Cell nucleus
Cell Nucleus - enzymology
Cells
Cellular biology
Cellular immunity
Cytosol - enzymology
Delta cells
Enzymes
Fluorescent Antibody Technique
Hot Temperature
Mice
Mice, Inbred BALB C
Microscopy, Confocal
NIH 3T3 cells
Protein Kinase C
Protein Processing, Post-Translational
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - metabolism
Rats
Shock heating
Sodium
Sodium Compounds - pharmacology
Stress, Physiological
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Summary:Effects of environmental stresses on the subcellular localization of PKN were investigated in NIH 3T3, BALB/c 3T3, and Rat-1 cells. The immunofluorescence of PKN resided prominently in the cytoplasmic region in nonstressed cells. When these cells were treated at 42 degrees C, there was a time-dependent decrease of the immunofluorescence of PKN in the cytoplasmic region that correlated with an increase within the nucleus as observed by confocal microscope. After incubation at 37 degrees C following heat shock, the immunofluorescence of PKN returned to the perinuclear and cytoplasmic regions from the nucleus. The nuclear translocation of PKN by heat shock was supported by the biochemical subcellular fractionation and immunoblotting. The nuclear localization of PKN was also observed when the cells were exposed to other stresses such as sodium arsenite and serum starvation. These results raise the possibility that there is a pathway mediating stress signals from the cytosol to the nucleus through PKN.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.19.10195