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Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions

Histone H1, a major structural component of chromatin fiber, is believed to act as a general repressor of transcription. To investigate in vivo the role of this protein in transcription regulation during development of a multicellular organism, we made transgenic tobacco plants that overexpress the...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1996-09, Vol.93 (19), p.10250-10255
Main Authors: Prymakowska-Bosak, M. (Warsaw University, Warsaw, Poland.), Przewloka, M.R, Iwkiewicz, J, Egierszdorff, S, Kuras, M, Chaubet, N, Gigot, C, Spiker, S, Jerzmanowski, A
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container_title Proceedings of the National Academy of Sciences - PNAS
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creator Prymakowska-Bosak, M. (Warsaw University, Warsaw, Poland.)
Przewloka, M.R
Iwkiewicz, J
Egierszdorff, S
Kuras, M
Chaubet, N
Gigot, C
Spiker, S
Jerzmanowski, A
description Histone H1, a major structural component of chromatin fiber, is believed to act as a general repressor of transcription. To investigate in vivo the role of this protein in transcription regulation during development of a multicellular organism, we made transgenic tobacco plants that overexpress the gene for Arabidopsis histone H1. In all plants that overexpressed H1 the total H1-to-DNA ratio in chromatin increased 2.3-2.8 times compared with the physiological level. This was accompanied by 50-100% decrease of native tobacco H1. The phenotypic changes in H1-overexpressing plants ranged from mild to severe perturbations in morphological appearance and flowering. No correlation was observed between the extent of phenotypic change and the variation in the amount of overexpressed H1 or the presence or absence of the native tobacco H1. However, the severe phenotypic changes were correlated with early occurrence during plant growth of cells with abnormally heterochromatinized nuclei. Such cells occurred considerably later in plants with milder changes. Surprisingly, the ability of cells with highly heterochromatinized nuclei to fulfill basic physiological functions, including differentiation, was not markedly hampered. The results support the suggestion that chromatin structural changes dependent on H1 stoichiometry and on the profile of major H1 variants have limited regulatory effect on the activity of genes that control basal cellular functions. However, the H1-mediated chromatin changes can be of much greater importance for the regulation of genes involved in control of specific developmental programs.
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(Warsaw University, Warsaw, Poland.) ; Przewloka, M.R ; Iwkiewicz, J ; Egierszdorff, S ; Kuras, M ; Chaubet, N ; Gigot, C ; Spiker, S ; Jerzmanowski, A</creator><creatorcontrib>Prymakowska-Bosak, M. (Warsaw University, Warsaw, Poland.) ; Przewloka, M.R ; Iwkiewicz, J ; Egierszdorff, S ; Kuras, M ; Chaubet, N ; Gigot, C ; Spiker, S ; Jerzmanowski, A</creatorcontrib><description>Histone H1, a major structural component of chromatin fiber, is believed to act as a general repressor of transcription. To investigate in vivo the role of this protein in transcription regulation during development of a multicellular organism, we made transgenic tobacco plants that overexpress the gene for Arabidopsis histone H1. In all plants that overexpressed H1 the total H1-to-DNA ratio in chromatin increased 2.3-2.8 times compared with the physiological level. This was accompanied by 50-100% decrease of native tobacco H1. 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subjects ADN
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
Arabidopsis
Arabidopsis - metabolism
ARABIDOPSIS THALIANA
Biology
Cell Nucleus - ultrastructure
Chromatin
Chromatin - ultrastructure
CHROMATINE
Complementary DNA
CROMATINA
DNA
DNA, Plant - metabolism
ESTRUCTURA CELULAR
EXPRESION GENICA
EXPRESSION DES GENES
FLORACION
FLORAISON
Flowering
Gels
GENE
GENES
Genes, Plant
Heterochromatin - ultrastructure
HISTONAS
HISTONE
Histones
Histones - biosynthesis
Histones - genetics
Leaves
Nicotiana - growth & development
Nicotiana - physiology
NICOTIANA TABACUM
Nucleosomes - ultrastructure
Phenotype
Phenotypes
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants
Plants, Genetically Modified
Plants, Toxic
Proteins
STRUCTURE CELLULAIRE
Tobacco
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRASTORNOS DEL CRECIMIENTO
TROUBLE DE LA CROISSANCE
ULTRAESTRUCTURA
ULTRASTRUCTURE
title Histone H1 overexpressed to high level in tobacco affects certain developmental programs but has limited effect on basal cellular functions
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