In vitro translation of the full-length RNA transcript of figwort mosaic virus (caulimovirus)

The circular DNA genome of FMV consists of seven tandemly arranged genes placed successively on a full-length RNA transcript that spans the entire circular viral genome. This transcript is a tentative mRNA for at least five of the six major conserved genes of this virus (genes I-V) that are position...

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Bibliographic Details
Published in:Gene expression 1996-01, Vol.5 (3), p.143-153
Main Authors: Ranu, R.S. (Colorado State University, Fort Collins, CO.), Gowda, S, Scholthof, H, Wu, F.C, Shepherd, R.J
Format: Article
Language:English
Subjects:
ACILTRANSFERASA
ACYLTRANSFERASE
ACYLTRANSFERASES
Animals
ARN MENSAJERO
ARN MESSAGER
Base Sequence
caulimovirus
Caulimovirus - genetics
CHLORAMPHENICOL ACETYLTRANSFERASE
CISTRONS
CONEJO (ORYCTOLAGUS)
ERITROCITOS
ERYTHROCYTE
ERYTHROCYTES
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
figwort mosaic virus
Figwort Mosaic Virus (fmv)
GENE
GENES
IN VITRO EXPERIMENTATION
In Vitro Translation
LAPIN (ORYCTOLAGUS)
MESSENGER RNA
Molecular Sequence Data
PLANT VIRUSES
POLYCISTRONIC MESSENGER RNA
Polycistronic Mrna
Protein Biosynthesis
PROTEINAS
PROTEINE
PROTEINS
RABBITS
REPORTER GENES
Reticulocyte Lysates
RETICULOCYTES
RNA, Viral
STRUCTURAL GENES
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Description
Summary:The circular DNA genome of FMV consists of seven tandemly arranged genes placed successively on a full-length RNA transcript that spans the entire circular viral genome. This transcript is a tentative mRNA for at least five of the six major conserved genes of this virus (genes I-V) that are positioned on this transcript. The sixth major gene (gene VI) is expressed as a separate monocistronic transcript. A long 5′-nontranslated leader (598 nucleotides), a small nonconserved gene (VII), and a short intergenic region (57 nucleotides) precede the five major conserved genes (I through V) on the full-length transcript. A reporter gene (CAT), as a separate cistron or fused in-frame, to viral cistrons in various downstream positions in cloned versions of the viral genome was used in a transcription vector to generate artificial full-length transcripts of FMV. When these mRNAs were translated in vitro (rabbit reticulocyte lysate system), the reporter gene was translated efficiently in all positions. Translation of internal native viral gene positioned on the full-length transcript of FMV was also determined (the gene VI product). These observations suggest that the full-length FMV transcript functions as a polycistronic mRNA in plants. Results are best explained on the basis of translational coupling/relay race model.
ISSN:1052-2166
1555-3884