Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection

A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C 8 column(250 × 4.6mm I.D.), using an isocratic eluent consisting of anacetonitrile-0.02 M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-02, Vol.677 (1), p.167-171
Main Authors: Gigosos, Pilar Gonza´lez, Ferna´ndez, Teresa Ferna´ndez, Mari´z, Oliva Cadahi´a, Fente Sampayo, Cristina A., Abui´n, Carlos Franco, Sa´ez, Alberto Cepeda
Format: Article
Language:English
Subjects:
Animals
Aqueous Humor - chemistry
Calibration
Chromatography, High Pressure Liquid
Clenbuterol
Clenbuterol - chemistry
Drug Residues - chemistry
Rats
Retina - chemistry
Spectrophotometry, Ultraviolet
Ultracentrifugation
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Summary:A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C 8 column(250 × 4.6mm I.D.), using an isocratic eluent consisting of anacetonitrile-0.02 M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid. The method was linear from 2.5 to 50 ng injected. The detection limit was established to be 0.5 ng (signal/background ratio: 3), and the quantification limit was 2.5 ng. With the proposed method, we got a simple and rapid detection of clenbuterol in the retina, part of the animal where the biggest amount of clenbuterol is accumulated and where it remains for the longest time after any treatment.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(95)00476-9