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Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection
A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C 8 column(250 × 4.6mm I.D.), using an isocratic eluent consisting of anacetonitrile-0.02 M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid...
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| Published in: | Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-02, Vol.677 (1), p.167-171 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c386t-cb87f47bd2f128a90c3e3c0f34d2d90e04e6d3f40734ffe39ec35b21ea0151443 |
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| cites | cdi_FETCH-LOGICAL-c386t-cb87f47bd2f128a90c3e3c0f34d2d90e04e6d3f40734ffe39ec35b21ea0151443 |
| container_end_page | 171 |
| container_issue | 1 |
| container_start_page | 167 |
| container_title | Journal of chromatography. B, Biomedical applications |
| container_volume | 677 |
| creator | Gigosos, Pilar Gonza´lez Ferna´ndez, Teresa Ferna´ndez Mari´z, Oliva Cadahi´a Fente Sampayo, Cristina A. Abui´n, Carlos Franco Sa´ez, Alberto Cepeda |
| description | A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C
8 column(250 × 4.6mm I.D.), using an isocratic eluent consisting of anacetonitrile-0.02
M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid. The method was linear from 2.5 to 50 ng injected. The detection limit was established to be 0.5 ng (signal/background ratio: 3), and the quantification limit was 2.5 ng. With the proposed method, we got a simple and rapid detection of clenbuterol in the retina, part of the animal where the biggest amount of clenbuterol is accumulated and where it remains for the longest time after any treatment. |
| doi_str_mv | 10.1016/0378-4347(95)00476-9 |
| format | article |
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M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid. The method was linear from 2.5 to 50 ng injected. The detection limit was established to be 0.5 ng (signal/background ratio: 3), and the quantification limit was 2.5 ng. With the proposed method, we got a simple and rapid detection of clenbuterol in the retina, part of the animal where the biggest amount of clenbuterol is accumulated and where it remains for the longest time after any treatment.</description><identifier>ISSN: 0378-4347</identifier><identifier>ISSN: 1572-6495</identifier><identifier>DOI: 10.1016/0378-4347(95)00476-9</identifier><identifier>PMID: 8925091</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Aqueous Humor - chemistry ; Calibration ; Chromatography, High Pressure Liquid ; Clenbuterol ; Clenbuterol - chemistry ; Drug Residues - chemistry ; Rats ; Retina - chemistry ; Spectrophotometry, Ultraviolet ; Ultracentrifugation</subject><ispartof>Journal of chromatography. B, Biomedical applications, 1996-02, Vol.677 (1), p.167-171</ispartof><rights>1996 Elsevier Science B.V. All rights reserved</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-cb87f47bd2f128a90c3e3c0f34d2d90e04e6d3f40734ffe39ec35b21ea0151443</citedby><cites>FETCH-LOGICAL-c386t-cb87f47bd2f128a90c3e3c0f34d2d90e04e6d3f40734ffe39ec35b21ea0151443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8925091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gigosos, Pilar Gonza´lez</creatorcontrib><creatorcontrib>Ferna´ndez, Teresa Ferna´ndez</creatorcontrib><creatorcontrib>Mari´z, Oliva Cadahi´a</creatorcontrib><creatorcontrib>Fente Sampayo, Cristina A.</creatorcontrib><creatorcontrib>Abui´n, Carlos Franco</creatorcontrib><creatorcontrib>Sa´ez, Alberto Cepeda</creatorcontrib><title>Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection</title><title>Journal of chromatography. B, Biomedical applications</title><addtitle>J Chromatogr B Biomed Appl</addtitle><description>A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C
8 column(250 × 4.6mm I.D.), using an isocratic eluent consisting of anacetonitrile-0.02
M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid. The method was linear from 2.5 to 50 ng injected. The detection limit was established to be 0.5 ng (signal/background ratio: 3), and the quantification limit was 2.5 ng. With the proposed method, we got a simple and rapid detection of clenbuterol in the retina, part of the animal where the biggest amount of clenbuterol is accumulated and where it remains for the longest time after any treatment.</description><subject>Animals</subject><subject>Aqueous Humor - chemistry</subject><subject>Calibration</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Clenbuterol</subject><subject>Clenbuterol - chemistry</subject><subject>Drug Residues - chemistry</subject><subject>Rats</subject><subject>Retina - chemistry</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Ultracentrifugation</subject><issn>0378-4347</issn><issn>1572-6495</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhnNQ_Fj9Bwo56qGaNOm2uQiy-AULgug5pMnERtqmJu3K_gD_t1lX9uhphpl55515EDqj5IoSOr8mrKwyznh5IYpLQng5z8QeOtqVD9FxjB-EME7y_AAdVCIviKBH6PtFDc5g1RscXTe0gA2MEDrXq9H5HnuLdQt9PaWib3GA6MwEEbs-5WOawvUaN-69yQYI1odO9Rpw6z6ntFU3wXdq9O9BDc0af7mxwVM7BrVyvoXx10pvbE7QvlVthNO_OENv93evi8ds-fzwtLhdZppV8zHTdVVaXtYmtzSvlCCaAdPEMm5yIwgQDnPDLCcl49YCE6BZUecUFKEF5ZzNEN_u1cHHGMDKIbhOhbWkRG5Ayg0xuSEmRSF_QUqRZOdb2TDVHZid6I9i6t9s-5BuXzkIMmoHCYRxIT0ojXf_G_wAUG2Iew</recordid><startdate>19960223</startdate><enddate>19960223</enddate><creator>Gigosos, Pilar Gonza´lez</creator><creator>Ferna´ndez, Teresa Ferna´ndez</creator><creator>Mari´z, Oliva Cadahi´a</creator><creator>Fente Sampayo, Cristina A.</creator><creator>Abui´n, Carlos Franco</creator><creator>Sa´ez, Alberto Cepeda</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19960223</creationdate><title>Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection</title><author>Gigosos, Pilar Gonza´lez ; Ferna´ndez, Teresa Ferna´ndez ; Mari´z, Oliva Cadahi´a ; Fente Sampayo, Cristina A. ; Abui´n, Carlos Franco ; Sa´ez, Alberto Cepeda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-cb87f47bd2f128a90c3e3c0f34d2d90e04e6d3f40734ffe39ec35b21ea0151443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Aqueous Humor - chemistry</topic><topic>Calibration</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Clenbuterol</topic><topic>Clenbuterol - chemistry</topic><topic>Drug Residues - chemistry</topic><topic>Rats</topic><topic>Retina - chemistry</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Ultracentrifugation</topic><toplevel>online_resources</toplevel><creatorcontrib>Gigosos, Pilar Gonza´lez</creatorcontrib><creatorcontrib>Ferna´ndez, Teresa Ferna´ndez</creatorcontrib><creatorcontrib>Mari´z, Oliva Cadahi´a</creatorcontrib><creatorcontrib>Fente Sampayo, Cristina A.</creatorcontrib><creatorcontrib>Abui´n, Carlos Franco</creatorcontrib><creatorcontrib>Sa´ez, Alberto Cepeda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of chromatography. B, Biomedical applications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gigosos, Pilar Gonza´lez</au><au>Ferna´ndez, Teresa Ferna´ndez</au><au>Mari´z, Oliva Cadahi´a</au><au>Fente Sampayo, Cristina A.</au><au>Abui´n, Carlos Franco</au><au>Sa´ez, Alberto Cepeda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection</atitle><jtitle>Journal of chromatography. B, Biomedical applications</jtitle><addtitle>J Chromatogr B Biomed Appl</addtitle><date>1996-02-23</date><risdate>1996</risdate><volume>677</volume><issue>1</issue><spage>167</spage><epage>171</epage><pages>167-171</pages><issn>0378-4347</issn><issn>1572-6495</issn><abstract>A new method for the determination of clenbuterol by reversed-phase HPLC with UV detection has been developed. Clenbuterol was eluted on a C
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M phosphate buffer (25:75, v/v) adjusted to pH 2.8 with phosphoric acid. The method was linear from 2.5 to 50 ng injected. The detection limit was established to be 0.5 ng (signal/background ratio: 3), and the quantification limit was 2.5 ng. With the proposed method, we got a simple and rapid detection of clenbuterol in the retina, part of the animal where the biggest amount of clenbuterol is accumulated and where it remains for the longest time after any treatment.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8925091</pmid><doi>10.1016/0378-4347(95)00476-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4347 |
| ispartof | Journal of chromatography. B, Biomedical applications, 1996-02, Vol.677 (1), p.167-171 |
| issn | 0378-4347 1572-6495 |
| language | eng |
| recordid | cdi_pubmed_primary_8925091 |
| source | ScienceDirect Freedom Collection 2022-2024 |
| subjects | Animals Aqueous Humor - chemistry Calibration Chromatography, High Pressure Liquid Clenbuterol Clenbuterol - chemistry Drug Residues - chemistry Rats Retina - chemistry Spectrophotometry, Ultraviolet Ultracentrifugation |
| title | Rapid and simple determination of clenbuterol residues in retina by high-performance liquid chromatography with ultraviolet detection |
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