In Vitro Studies of Chemical Mutagens and Carcinogens. I. Stability Studies in Cell Culture Medium

A quantitative microbial mutagenesis assay was used to study the stability of known mutagenic and carcinogenic compounds in cell culture medium. Ten direct-acting carcinogens, when incubated in culture medium with 15% fetal bovine serum at pH 7-2-7-4 and 37° C, became inactive at varying rates. Biol...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1977-09, Vol.59 (3), p.941-944
Main Authors: Jensen, Erling M., LaPolla, Robert J., Kirby, Paul E., Haworth, Steve R.
Format: Article
Language:English
Subjects:
Carcinogens
Culture Media
Half-Life
In Vitro Techniques
Mutagens
Salmonella - drug effects
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Summary:A quantitative microbial mutagenesis assay was used to study the stability of known mutagenic and carcinogenic compounds in cell culture medium. Ten direct-acting carcinogens, when incubated in culture medium with 15% fetal bovine serum at pH 7-2-7-4 and 37° C, became inactive at varying rates. Biologic half-lives of the test compounds ranged from 8 minutes to 67 hours. In contrast, six procarcinogens showed no significant inactivation after 3 weeks' incubation. The biologic half-lives of each compound were presented, and the significance of these findings as they relate to cell culture carcinogenesis and mutagenesis assays was discussed.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/59.3.941