High-performance liquid chromatographic assay for melphalan in human plasma Application to pharmacokinetic studies

This paper describes a simple, rapid and reproducible high-performance liquid chromatographic method (HPLC) with ultraviolet absorbance detection for the analysis of melphalan in plasma. The HPLC column was an Ultrasphere ODS (5 μm) and the eluent was composed of methanol, purified water and acetic...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1996-11, Vol.686 (1), p.43-49
Main Authors: Pinguet, Frédéric, Joulia, Jean Michel, Martel, Pascal, Grosse, Pierre Yves, Astre, Cécile, Bressolle, Françoise
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Alkylating - administration & dosage
Antineoplastic Agents, Alkylating - blood
Antineoplastic Agents, Alkylating - chemistry
Antineoplastic Agents, Alkylating - pharmacokinetics
Chromatography, High Pressure Liquid - methods
Circadian Rhythm
Drug Stability
Humans
Injections, Intravenous
Linear Models
Melphalan
Melphalan - administration & dosage
Melphalan - blood
Melphalan - chemistry
Melphalan - pharmacokinetics
Parabens - analysis
Parabens - chemistry
Pharmacokinetics
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
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Summary:This paper describes a simple, rapid and reproducible high-performance liquid chromatographic method (HPLC) with ultraviolet absorbance detection for the analysis of melphalan in plasma. The HPLC column was an Ultrasphere ODS (5 μm) and the eluent was composed of methanol, purified water and acetic acid (49.5:49.5:1, v/v). The detection was performed at 261 nm. The method involved a simple treatment of the samples with methanol. The propylparaben was used as internnal standard. Linear detection response was obtained for concentrations ranging from 50 to 2500 ng/ml. Recovery from plasma proved to be more than 90%. Precision, expressed as C.V., was in the 0.5 to 9% range. Accuracy ranged from 95 to 102%. This method was used to determine the pharmacokinetic parameters of melphalan following high-dose (140 mg/m 2) intravenous administration in patients with advanced malignancies undergoing peripheral blood hematopoietic progenitor-cell transplantation.
ISSN:0378-4347
1572-6495
DOI:10.1016/S0378-4347(96)00184-3