Biochemical Studies on the Toxicity of 1, 1′-Dimethyl-4, 4′-Bipyridylium Dichloride in the Rat

The effect of intraperitoneal administration of lethal dose (50 mg/kg) of paraquat on the microsomal cysteine levels in the plasma, liver and lung of adult male Wistar rats has been investigated using Rank Chromaspek amino acid analyzer. The microsomal alanine levels were also determined to help in...

Full description

Saved in:
Bibliographic Details
Published in:Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Comparative pharmacology and toxicology, 1997-05, Vol.117 (1), p.103-109
Main Authors: Nwabisi, V.C, Nwanze, E.A.C
Format: Article
Language:English
Subjects:
Alanine - blood
Alanine - metabolism
Analysis of Variance
Animals
Buthionine Sulfoximine - administration & dosage
Buthionine Sulfoximine - pharmacology
Buthionine,-[S,R]sulfoximine
Cysteine - blood
Cysteine - metabolism
diethylmaleate
Herbicides - administration & dosage
Herbicides - analysis
Herbicides - toxicity
Lung - ultrastructure
Male
Maleates - administration & dosage
Maleates - pharmacology
microsomes
Microsomes - chemistry
Microsomes, Liver - chemistry
NADPH–cytochrome P–450 reductase
paraquat
Paraquat - administration & dosage
Paraquat - analysis
Paraquat - toxicity
rank chromaspek
Rats
Rats, Wistar
S- β-(4-pyridly-ethyl) L-cysteine
toxicity
γ-glutamyl cysteine synthetase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of intraperitoneal administration of lethal dose (50 mg/kg) of paraquat on the microsomal cysteine levels in the plasma, liver and lung of adult male Wistar rats has been investigated using Rank Chromaspek amino acid analyzer. The microsomal alanine levels were also determined to help in assessing the extent of paraquat interference with cellular protein. DL–Buthionine–[S,R]–Sulfoximine (BSO) and Diethyl maleate (DEM) were used to potentiate the toxic effect of the bipyridyl. The microsomal cysteine levels were significantly (P ≤ 0.05) depressed in the plasma, liver and lung of the paraquat-treated rats compared with the saline-injected group but the alanine levels were not similarly affected. Probably, paraquat poisoning interferes specifically with the cellular cysteine content in the rat. These findings could provide a valuable information on the biochemical mechanism of paraquat intoxication.
ISSN:0742-8413
1367-8280
DOI:10.1016/S0742-8413(96)00166-1