Water Immiscible Solvent Based Immunoassay

A competitive monoclonal antibody-based immunoassay which quantifies a hydrophobic hapten (R x ) in water immiscible solvents, obviating the need of a pre-extraction step, has been developed. Approximately linear dose response profiles of analyte, over the range 1-20 ugml −1 in the hydrophobic solve...

Full description

Saved in:
Bibliographic Details
Published in:Journal of immunoassay (Monticello, N.Y.) N.Y.), 1997-08, Vol.18 (3), p.235-246
Main Authors: Aston, J. Paul, Hitchings, Eric J., Ball, R. Lathan, Weeks, Ian, Woodhead, J. Stuart
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Dose-Response Relationship, Immunologic
Enzyme-Linked Immunosorbent Assay - methods
hapten immunoassay
Haptens - immunology
Humans
Monoclonal antibody
organic solvent
Serum Albumin - chemistry
Solubility
Solvents
Ultraviolet Rays
Water
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A competitive monoclonal antibody-based immunoassay which quantifies a hydrophobic hapten (R x ) in water immiscible solvents, obviating the need of a pre-extraction step, has been developed. Approximately linear dose response profiles of analyte, over the range 1-20 ugml −1 in the hydrophobic solvents, hexane, toluene and xylene were obtained. UV spectrophotometric analyses of R x dosed hexane confirm the phenomenon of antibody-mediated transfer of analyte from the organic to the aqueous milieu. Preliminary data on the effect of water immiscible solvents on the immunoreactivity of a monoclonal antibody in free solution are presented. The potential industrial applications of water immiscible solvent based immunoassays are discussed.
ISSN:0197-1522
DOI:10.1080/01971529708005817