Compensation for D-glutamate auxotrophy of Escherichia coli WM335 by D-amino acid aminotransferase gene and regulation of murI expression

D-Glutamate, an indispensable component of peptidoglycane of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests the presence of a...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-01, Vol.62 (1), p.193-195
Main Authors: Liu, L. (Kyoto Univ. (Japan)), Yoshimura, T, Endo, K, Kishimoto, K, Fuchikami, Y, Manning, J.M, Esaki, N, Soda, K
Format: Article
Language:English
Subjects:
ACIDE GLUTAMIQUE
ACIDO GLUTAMICO
Alanine Transaminase - biosynthesis
Alanine Transaminase - genetics
Alanine Transaminase - metabolism
Amino Acid Isomerases - biosynthesis
Amino Acid Isomerases - genetics
Amino Acid Isomerases - metabolism
AMINOTRANSFERASAS
AMINOTRANSFERASE
AMINOTRANSFERASES
Asparagine - genetics
Asparagine - metabolism
AUXOTROFIA
AUXOTROPHIE
AUXOTROPHY
Bacteriology
Biological and medical sciences
Culture Media - metabolism
Culture Media - pharmacology
D-Alanine Transaminase
D-amino acid aminotransferase
ESCHERICHIA COLI
Escherichia coli - metabolism
expression
Fundamental and applied biological sciences. Psychology
Gene Expression
Genetics
glutamate racemase
GLUTAMIC ACID
Glutamic Acid - metabolism
Glutamic Acid - pharmacology
ISOMERASAS
ISOMERASE
ISOMERASES
Lysine - genetics
Lysine - metabolism
Microbiology
murI
Mutagenesis, Site-Directed
regulation
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Summary:D-Glutamate, an indispensable component of peptidoglycane of bacteria, is provided by glutamate racemase in E. coli cells. Compensation for D-glutamate auxotrophy of E. coli WM335 cells lacking the glutamate racemase gene, murI, with the D-amino acid aminotransferase gene suggests the presence of a threshold concentration for the D-glutamate required by E. coli cells, as well as a regulation system for murI expression
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.62.193