The Saccharomyces cerevisiae SCS2 gene product, a homolog of a synaptobrevin-associated protein, is an integral membrane protein of the endoplasmic reticulum and is required for inositol metabolism

The Saccharomyces cerevisiae SCS2 gene has been cloned as a suppressor of inositol auxotrophy of CSE1 and hac1/ire16 mutants (J. Nikawa, A. Murakami, E. Esumi, and K. Hosaka, J. Biochem. 118:39-45, 1995) and has homology with a synaptobrevin/VAMP-associated protein, VAP-33, cloned from Aplysia calif...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Bacteriology 1998-04, Vol.180 (7), p.1700-1708
Main Authors: Kagiwada, S. (Nara Women's University, Nara, Japan.), Hosaka, K, Murata, M, Nikawa, J, Takatsuki, A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
Bacteriology
BIOLOGICAL DIFFERENCES
CELL MEMBRANES
CHEMICAL COMPOSITION
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Conserved Sequence
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
ENDOPLASMIC RETICULUM
Endoplasmic Reticulum - chemistry
Eukaryotic Cells
Fungal Proteins - analysis
Fungal Proteins - physiology
IMMUNOCYTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
Inositol - metabolism
MEMBRANAS CELULARES
MEMBRANE CELLULAIRE
Membrane Proteins - analysis
Membrane Proteins - physiology
Metabolism
Molecular biology
MOLECULAR SEQUENCE DATA
Molecular Weight
MUTANT
MUTANTES
MUTANTS
PROTEINAS
PROTEINE
PROTEINS
R-SNARE Proteins
RETICULO ENDOPLASMATICO
RETICULUM ENDOPLASMIQUE
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - metabolism
SPECIES DIFFERENCES
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Saccharomyces cerevisiae SCS2 gene has been cloned as a suppressor of inositol auxotrophy of CSE1 and hac1/ire16 mutants (J. Nikawa, A. Murakami, E. Esumi, and K. Hosaka, J. Biochem. 118:39-45, 1995) and has homology with a synaptobrevin/VAMP-associated protein, VAP-33, cloned from Aplysia californica (P. A. Skehel, K. C. Martin, E. R. Kandel, and D. Bartsch, Science 269:1580-1583, 1995). In this study we have characterized an SCS2 gene product (Scs2p). The product has a molecular mass of 35 kDa and is C-terminally anchored to the endoplasmic reticulum, with the bulk of the protein located in the cytosol. The disruption of the SCS2 gene causes yeast cells to exhibit inositol auxotrophy at temperatures of above 34 degrees C. Genetic studies reveal that the overexpression of the INO1 gene rescues the inositol auxotrophy of the SCS2 disruption strain. The significant primary structural feature of Scs2p is that the protein contains the 16-amino-acid sequence conserved in yeast and mammalian cells. The sequence is required for normal Scs2p function, because a mutant Scs2p that lacks the sequence does not complement the inositol auxotrophy of the SCS2 disruption strain. Therefore, the Scs2p function might be conserved among eukaryotic cells
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.180.7.1700-1708.1998