Chromosomal Instability in the Descendants of Unirradiated Surviving Cells after α -Particle Irradiation

We have demonstrated chromosomal instability in the clonal descendants of hemopoietic stem cells after irradiating murine bone marrow with α -particles. However, because cells that are irradiated by α -particles are defined by a Poisson distribution of individual particle traversals, there is an ine...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-05, Vol.95 (10), p.5730-5733
Main Authors: Lorimore, S. A., Kadhim, M. A., Pocock, D. A., Papworth, D., Stevens, D. L., Goodhead, D. T., Wright, E. G.
Format: Article
Language:English
Subjects:
Abnormalities, Radiation-Induced - genetics
Alpha Particles - adverse effects
Animals
Binomials
Biological Sciences
Bone marrow
Bone marrow cells
Cellular biology
Chromosomal instability
Chromosomes - radiation effects
Clone Cells - radiation effects
Descendants
Dose-Response Relationship, Radiation
Expected values
Genes
Genetics
Hematopoietic Stem Cells - radiation effects
Irradiation
Male
Mice
Radiation
Radiation dosage
Radionuclides
Radon
Stem cells
Studies
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Summary:We have demonstrated chromosomal instability in the clonal descendants of hemopoietic stem cells after irradiating murine bone marrow with α -particles. However, because cells that are irradiated by α -particles are defined by a Poisson distribution of individual particle traversals, there is an inevitable proportion of unirradiated cells in the surviving population. The calculated expected proportions of irradiated and nonirradiated cells indicate that the number of clonogenic cells transmitting chromosomal instability is greater than the number expected to be hit and survive. To investigate further this discrepancy, we studied the effects of interposing a grid between the cells and the α -particle source so that the surviving population consists predominantly of untraversed stem cells. Comparison with the same irradiation conditions without the grid reveals that the same level of instability is induced. The data confirm that α -particles induce chromosomal instability but instability is demonstrated in the progeny of nonirradiated stem cells and must be due to unexpected interactions between irradiated and non-irradiated cells. This untargeted effect has important implications for mechanistic studies of radiation action and for assessment of radiation risk.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.10.5730