Changes in Glycoconjugate Expression of the Sinus Mucosa during Experimental Sinusitis: A Lectin Histochemical Study of the Epithelium and Goblet Cell Development

Lectin expression in both normal and inflamed sinus mucosa in rabbit was investigated histochemically. A different staining pattern was observed in the inflamed mucosa, i.e. the degree of staining reactivity with Canavalia ensiformis (ConA) decreased, whereas staining with Ulex europaeus agglutinin-...

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Bibliographic Details
Published in:Acta oto-laryngologica 1998, Vol.118 (2), p.248-256
Main Author: NOBUYOSHI OTORI, BENGT CARLSÖÖ, PONTUS STIERNA
Format: Article
Language:English
Subjects:
Animals
Arachis
Biological and medical sciences
Canavalia ensiformis
Concanavalin A - metabolism
Ent. Stomatology
Female
Glycoconjugates - metabolism
Histocytochemistry
Investigative techniques, diagnostic techniques (general aspects)
Lectins - metabolism
Male
Medical sciences
Medicin och hälsovetenskap
Nasal Mucosa - cytology
Nasal Mucosa - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Peanut Agglutinin - metabolism
Plant Lectins
Rabbits
Sinusitis - metabolism
Time Factors
Triticum
Ulex europaeus
Wheat Germ Agglutinins - metabolism
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Summary:Lectin expression in both normal and inflamed sinus mucosa in rabbit was investigated histochemically. A different staining pattern was observed in the inflamed mucosa, i.e. the degree of staining reactivity with Canavalia ensiformis (ConA) decreased, whereas staining with Ulex europaeus agglutinin-I (UEA-I), Arachis hypoga (PNA) with neuraminidase pretreatment and Triticum vulgaris (WGA) intensified, indicating an enhanced fucosylation as well as sialylation. Goblet cells were stained with UEA-I, WGA, PNA and PNA with neuraminidase pretreatment, but scarcely with ConA. Positive PNA staining of basal cells and epithelial secretory granules was observed in the inflamed mucosa, especially close to areas where goblet cell development was prolific. It was therefore assumed that basal cells can differentiate into goblet cells-through epithelial secretory cells-accompanied by sialylation and/or sulphonation of their mucins. It is concluded that the changes in glycoconjugate expression as well as goblet cell development in the inflamed mucosa are of importance for both local host resistance and defence mechanisms against microorganisms during the early stages of the inflammatory process.
ISSN:0001-6489
1651-2251
DOI:10.1080/00016489850154982