Biological dynamics and distribution of 3-hydroxy fatty acids in the yeast Dipodascopsis uninucleata as investigated by immunofluorescence microscopy. Evidence for a putative regulatory role in the sexual reproductive cycle

Dipodascopsis uninucleata has been recently shown to produce 3-hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3-hydroxy fatty acids (3-OH-FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluoresce...

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Bibliographic Details
Published in:FEBS letters 1998-05, Vol.427 (3), p.345-348
Main Authors: Kock, J.L.F, Venter, P, Linke, D, Schewe, T, Nigam, S
Format: Article
Language:English
Subjects:
3-HETE
3-Hydroxy fatty acid
Animals
Antibodies - immunology
Ascomycota - metabolism
Ascomycota - physiology
Ascomycota - ultrastructure
Cross Reactions
Dipodascopsis uninucleata
Fatty Acids, Unsaturated - immunology
Fatty Acids, Unsaturated - metabolism
Fatty Acids, Unsaturated - physiology
Hydroxyeicosatetraenoic Acids - immunology
Immunofluorescence
Microscopy, Fluorescence
Rabbits
Reproduction
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Summary:Dipodascopsis uninucleata has been recently shown to produce 3-hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3-hydroxy fatty acids (3-OH-FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluorescence microscopy their occurrence in fixed cells with or without cell walls using an antibody raised against 3 R-hydroxy-5 Z,8 Z,11 Z,14 Z-eicosatetraenoic acid (3 R-HETE), the biotransformation product from arachidonic acid (AA). This antibody turned out to cross-react with other 3-OH-FA. 3-OH-FA were detected in situ in gametangia, asci, as well as between released ascospores, and proved to be associated with the sexual reproductive stage of the life cycle of the yeast. Acetylsalicylic acid (1 mM), which is known to suppress the formation of 3-OH-FA from exogenous polyenoic fatty acids, inhibited the occurrence of immunoreactive material as well as the sexual phase of the life cycle suggesting a prominent regulatory role of 3-OH-FA for the latter.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(98)00406-2