Biological dynamics and distribution of 3-hydroxy fatty acids in the yeast Dipodascopsis uninucleata as investigated by immunofluorescence microscopy. Evidence for a putative regulatory role in the sexual reproductive cycle

• Published in: FEBS letters 1998-05, Vol.427 (3), p.345-348
• Main Authors: Kock, J.L.F, Venter, P, Linke, D, Schewe, T, Nigam, S
• Format: Article
• Language: English
• Subjects: 3-HETE; 3-Hydroxy fatty acid; Animals; Antibodies - immunology; Ascomycota - metabolism; Ascomycota - physiology; Ascomycota - ultrastructure; Cross Reactions; Dipodascopsis uninucleata; Fatty Acids, Unsaturated - immunology; Fatty Acids, Unsaturated - metabolism; Fatty Acids, Unsaturated - physiology; Hydroxyeicosatetraenoic Acids - immunology; Immunofluorescence; Microscopy, Fluorescence; Rabbits; Reproduction
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)00406-2

Dipodascopsis uninucleata has been recently shown to produce 3-hydroxy polyenoic fatty acids from several exogenous polyenoic fatty acids. In order to examine whether endogenous 3-hydroxy fatty acids (3-OH-FA) may be implicated in the developmental biology of this yeast, we mapped by immunofluorescence microscopy their occurrence in fixed cells with or without cell walls using an antibody raised against 3 R-hydroxy-5 Z,8 Z,11 Z,14 Z-eicosatetraenoic acid (3 R-HETE), the biotransformation product from arachidonic acid (AA). This antibody turned out to cross-react with other 3-OH-FA. 3-OH-FA were detected in situ in gametangia, asci, as well as between released ascospores, and proved to be associated with the sexual reproductive stage of the life cycle of the yeast. Acetylsalicylic acid (1 mM), which is known to suppress the formation of 3-OH-FA from exogenous polyenoic fatty acids, inhibited the occurrence of immunoreactive material as well as the sexual phase of the life cycle suggesting a prominent regulatory role of 3-OH-FA for the latter.