mRNA coding for voltage-gated sodium channel beta2 subunit in rat central nervous system: cellular distribution and changes following kainate-induced seizures

The cellular distribution of sodium channel beta2 subunit mRNA was examined in the central nervous system from adult Wistar rats using a non-radioactive in situ hybridization method with digoxigenin-labeled cRNA probes. The expression of the subunit was strong in cerebral and cerebellar cortex, in m...

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Bibliographic Details
Published in:Neuroscience letters 1998-06, Vol.249 (1), p.53
Main Authors: Gastaldi, M, Robaglia-Schlupp, A, Massacrier, A, Planells, R, Cau, P
Format: Article
Language:English
Subjects:
Animals
Brain - anatomy & histology
Brain - cytology
Brain - metabolism
Calcium Channels - biosynthesis
Calcium Channels - genetics
Calcium Channels, L-Type
Central Nervous System - anatomy & histology
Central Nervous System - cytology
Central Nervous System - metabolism
Excitatory Amino Acid Agonists - toxicity
In Situ Hybridization
Ion Channel Gating
Kainic Acid - toxicity
Kinetics
Male
Rats
Rats, Wistar
RNA, Complementary - genetics
RNA, Messenger - biosynthesis
Seizures - chemically induced
Seizures - metabolism
Spinal Cord - cytology
Spinal Cord - metabolism
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Summary:The cellular distribution of sodium channel beta2 subunit mRNA was examined in the central nervous system from adult Wistar rats using a non-radioactive in situ hybridization method with digoxigenin-labeled cRNA probes. The expression of the subunit was strong in cerebral and cerebellar cortex, in medulla oblongata and in the spinal cord whereas heterogeneous in hippocampus. The distribution was evaluated in hippocampus and cerebral cortex from 1 to 72 h after kainate injection and compared to control rats using densitometric analysis. In these areas, a transient increase was seen 1 h after the drug administration, followed, in the hippocampus, by a significant decrease. These variations differ from those we previously reported for alpha subunits and might play a role in cellular excitability changes occurring in the course of seizures.
ISSN:0304-3940