Application of ion-exchange cartridge clean-up in food analysis: I. Simultaneous determination of thiabendazole and imazalil in citrus fruit and banana using high-performance liquid chromatography with ultraviolet detection

• Published in: Journal of Chromatography A 1998-06, Vol.810 (1), p.81-87
• Main Authors: Ito, Yuko, Ikai, Yoshitomo, Oka, Hisao, Hayakawa, Junko, Kagami, Tadaaki
• Format: Article
• Language: English
• Subjects: Anthelmintics - analysis; Calibration; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Citrus - chemistry; Food Analysis; Fungicides, Industrial - analysis; Hydrogen-Ion Concentration; Imazalil; Imidazoles - analysis; Indicators and Reagents; Spectrophotometry, Ultraviolet; Thiabendazole; Thiabendazole - analysis; Zingiberales - chemistry
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(98)00185-X

A simple, rapid and reproducible analytical method for thiabendazole (TBZ) and imazalil (IMA) in citrus fruit and banana has been developed. The method involves the use of an ion-exchange cartridge for sample clean-up followed by ion-pair high-performance liquid chromatography with ultraviolet detection. The recoveries of TBZ and IMA from citrus fruits spiked at levels of 10 μg/g and 5 μg/g were in the range of 94–98% and 93–98% with coefficients of variation of 0.5–2.2% and 1.6–2.7%, respectively. The recoveries of TBZ and IMA from banana spiked at levels of 3 μg/g and 2 μg/g were 94% and 94% with coefficients of variation 1.1% and 4.9%, respectively. The detection limits for TBZ and IMA were 0.1 μg/g in citrus fruit and 0.05 μg/g in banana.