Combinational effects of vitamin D3 and retinoic acid (all trans and 9 cis) on proliferation, differentiation, and programmed cell death in two small cell lung carcinoma cell lines

The effects of a combination of vitamin D3 [1,25(OH)2D3] and retinoic acid (RA) on proliferation, differentiation, and apoptosis of the human small cell lung carcinoma (SCLC) cell lines NCI-H82 and NCI-H209 were evaluated. Cell proliferation was inhibited by 1,25(OH)2D3 and RA alone. The combination...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1998-08, Vol.249 (3), p.735
Main Authors: Guzey, M. (University of Wisconsin, Madison, WI.), Sattler, C, Deluca, H.F
Format: Article
Language:English
Subjects:
APOPTOSE
APOPTOSIS
Apoptosis - drug effects
CARCINOMA
Carcinoma, Small Cell - drug therapy
Carcinoma, Small Cell - metabolism
Carcinoma, Small Cell - pathology
CARCINOME
Cell Differentiation - drug effects
Cell Division - drug effects
Cholecalciferol - administration & dosage
Drug Synergism
Drug Therapy, Combination
Humans
Lung Neoplasms - drug therapy
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Microscopy, Electron
RETINOL
Stereoisomerism
Thymidine - metabolism
Tretinoin - administration & dosage
Tumor Cells, Cultured
VITAMIN D
VITAMINA D
VITAMINE D
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Summary:The effects of a combination of vitamin D3 [1,25(OH)2D3] and retinoic acid (RA) on proliferation, differentiation, and apoptosis of the human small cell lung carcinoma (SCLC) cell lines NCI-H82 and NCI-H209 were evaluated. Cell proliferation was inhibited by 1,25(OH)2D3 and RA alone. The combination of 1,25(OH)2D3 and the cis form of retinoic acid resulted in an additive decrease in cell proliferation and the induction of apoptosis in various concentrations. Moreover, 3H-thymidine incorporation was inhibited and the number of viable cells was decreased. The characteristics of the apoptotic cells were examined and confirmed by morphologic analysis, light and electron microscopy, and fluorescence detection. It was concluded that 1,25(OH)2D3 and RA exert additive effects on the inhibition of proliferation and the induction of apoptosis in both the NCI-H82 and the NCI-H209 SCLC cell lines. This finding has important implications for the use of retinoids and 1,25(OH)2D3 in cancer prevention and in the therapy of small cell lung carcinoma
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1998.9186