Loading…

EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts

Several authors have alluded to the possible involvement of EDRF (NO) in ischemia-induced coronary artery dilation. Alternatively, it has been suggested that opening of ATP-dependent K channels could play a key role in this context. We studied the effects of sulfonylureas and NG-nitro-L-arginine (LN...

Full description

Saved in:
Bibliographic Details
Published in:Cardiovascular drugs and therapy 1998-07, Vol.12 (3), p.279-284
Main Authors: GASSER, R, KÖPPEL, H, BRUSSEE, H, GRISOLD, M, HOLZMANN, S, KLEIN, W
Format: Article
Language:English
Subjects:
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 284
container_issue 3
container_start_page 279
container_title Cardiovascular drugs and therapy
container_volume 12
creator GASSER, R
KÖPPEL, H
BRUSSEE, H
GRISOLD, M
HOLZMANN, S
KLEIN, W
description Several authors have alluded to the possible involvement of EDRF (NO) in ischemia-induced coronary artery dilation. Alternatively, it has been suggested that opening of ATP-dependent K channels could play a key role in this context. We studied the effects of sulfonylureas and NG-nitro-L-arginine (LNNA), a specific inhibitor of endothelial NO (EDRF) synthesis, on ischemia-induced coronary vasodilation in isolated Langendorff-perfused guinea pig hearts arrested with 15 mM KCl in normal Tyrode, and isolated pig coronary arteries precontracted with 43 mM KCl. In Isolated Langerdorff-perfused guinea pig heart, when hypoxia was simulated by switching 100% O2 in the perfusate to 100% N2, coronary perfusion pressure (CPP) fell from 90 cm H2O by 45 +/- 5 cm H2O. In the presence of LNNA, a specific inhibitor of NO synthetase in endothelial cells, CPP dropped by 44 +/- 6 cm H2O (n = 6; +/- SEM, no statistically significant). On biochemical simulation of ischemia (addition of iodoacetate [IAA]), CPP dropped 40 +/- 6 cm H2O, and in experiments performed under the same conditions but in the presence of LNNA, CPP dropped by 38 +/- 5 cm H2O (n = 6; +/- SEM; not statistically significant). When ischemia was simulated metabolically by equimolar replacement of 10 mM glucose with 2-deoxyglucose (DOG), an inhibitor of glycolysis CPP decreased by 24 +/- 1 cm H2O (n = 6; +/- SEM) after 15 minutes. This fall in CPP was almost prevented by 20 microM glibenclamide, whereas in the presence of 20 microM LNNA the DOG-induced decrease in CPP was not significantly inhibited, and CPP decreased by 22 +/- 2.6 cm H2O (n = 6; +/- SEM). In isolated pig coronary artery rings, maximal tension, achieved by depolarizing the smooth muscle cells by 43 mM KCl, decreased by 37 +/- 7% upon simulated hypoxia by replacing 100% O2 with 100% N2 in the perfusate (n = 6; +/- SEM) in arteries with intact endothelium. In arteries without endothelium, maximal tension also dropped by 35 +/- 6% (not statistically significant). In the same experiments the decrease in tension could be largely inhibited in the presence of 50 microM glibenclamide. Our results clearly show that in isolated perfused guinea pig hearts, as well as in isolated pig coronary arteries, EDRF does not play a decisive role in the coronary dilatory response to hypoxia and ischemia.
format article
fullrecord <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pubmed_primary_9784907</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>9784907</sourcerecordid><originalsourceid>FETCH-LOGICAL-p827-163b4de3bb450f99ca25fb7af986ccb5f30448ca9080d0d889abaa7b3446ba303</originalsourceid><addsrcrecordid>eNo9kM1KAzEUhQdRtP48gnAXbgOZyfwk7oq2KhYV6V5u_qaRNhmSGcFH8OnEN3JKS1cXzvk4h3uOskleNYw0RZkfZxMqCkpYQeuz7DylT0ppIwQ_zU5Fw0tBm0n2N7t_n4MOJoEPPWyMdtgbUCEGj_EbvjAF7dbYu-AhGRW83sp9gOQ2w6gbDS6pldk4hFuYQuoH_Q0j_DxdvoFaofdmnQC9hpffH-JdHwNZEIyt886bLXko60y0Q9o2ddGkNEQDzo_xYdezQN8ar0O0luzQUWyHMQVJ51pYGYx9usxOLK6Tudrfi2w5ny3vHsni9eHpbrogHS8aktdMltowKcuKWiEUFpWVDVrBa6VkZRktS65QUE411ZwLlIiNZGVZS2SUXWTXu9hukONqH110m_GHj_2yo3-z9zEpXNuIXrl0wPJK5HXN2T-8n4im</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts</title><source>Springer Link</source><creator>GASSER, R ; KÖPPEL, H ; BRUSSEE, H ; GRISOLD, M ; HOLZMANN, S ; KLEIN, W</creator><creatorcontrib>GASSER, R ; KÖPPEL, H ; BRUSSEE, H ; GRISOLD, M ; HOLZMANN, S ; KLEIN, W</creatorcontrib><description>Several authors have alluded to the possible involvement of EDRF (NO) in ischemia-induced coronary artery dilation. Alternatively, it has been suggested that opening of ATP-dependent K channels could play a key role in this context. We studied the effects of sulfonylureas and NG-nitro-L-arginine (LNNA), a specific inhibitor of endothelial NO (EDRF) synthesis, on ischemia-induced coronary vasodilation in isolated Langendorff-perfused guinea pig hearts arrested with 15 mM KCl in normal Tyrode, and isolated pig coronary arteries precontracted with 43 mM KCl. In Isolated Langerdorff-perfused guinea pig heart, when hypoxia was simulated by switching 100% O2 in the perfusate to 100% N2, coronary perfusion pressure (CPP) fell from 90 cm H2O by 45 +/- 5 cm H2O. In the presence of LNNA, a specific inhibitor of NO synthetase in endothelial cells, CPP dropped by 44 +/- 6 cm H2O (n = 6; +/- SEM, no statistically significant). On biochemical simulation of ischemia (addition of iodoacetate [IAA]), CPP dropped 40 +/- 6 cm H2O, and in experiments performed under the same conditions but in the presence of LNNA, CPP dropped by 38 +/- 5 cm H2O (n = 6; +/- SEM; not statistically significant). When ischemia was simulated metabolically by equimolar replacement of 10 mM glucose with 2-deoxyglucose (DOG), an inhibitor of glycolysis CPP decreased by 24 +/- 1 cm H2O (n = 6; +/- SEM) after 15 minutes. This fall in CPP was almost prevented by 20 microM glibenclamide, whereas in the presence of 20 microM LNNA the DOG-induced decrease in CPP was not significantly inhibited, and CPP decreased by 22 +/- 2.6 cm H2O (n = 6; +/- SEM). In isolated pig coronary artery rings, maximal tension, achieved by depolarizing the smooth muscle cells by 43 mM KCl, decreased by 37 +/- 7% upon simulated hypoxia by replacing 100% O2 with 100% N2 in the perfusate (n = 6; +/- SEM) in arteries with intact endothelium. In arteries without endothelium, maximal tension also dropped by 35 +/- 6% (not statistically significant). In the same experiments the decrease in tension could be largely inhibited in the presence of 50 microM glibenclamide. Our results clearly show that in isolated perfused guinea pig hearts, as well as in isolated pig coronary arteries, EDRF does not play a decisive role in the coronary dilatory response to hypoxia and ischemia.</description><identifier>ISSN: 0920-3206</identifier><identifier>EISSN: 1573-7241</identifier><identifier>PMID: 9784907</identifier><identifier>CODEN: CDTHET</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Adenosine Triphosphate - pharmacology ; Animals ; Biological and medical sciences ; Cardiology. Vascular system ; Coronary Circulation - drug effects ; Coronary heart disease ; Enzyme Inhibitors - therapeutic use ; Glyburide - pharmacology ; Guinea Pigs ; Heart ; In Vitro Techniques ; Medical sciences ; Myocardial Ischemia - chemically induced ; Myocardial Ischemia - physiopathology ; Nitric Oxide - physiology ; Nitroarginine - therapeutic use ; Nitrogen - pharmacology ; Perfusion ; Potassium Channels - physiology ; Vasodilation - physiology</subject><ispartof>Cardiovascular drugs and therapy, 1998-07, Vol.12 (3), p.279-284</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1591668$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9784907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GASSER, R</creatorcontrib><creatorcontrib>KÖPPEL, H</creatorcontrib><creatorcontrib>BRUSSEE, H</creatorcontrib><creatorcontrib>GRISOLD, M</creatorcontrib><creatorcontrib>HOLZMANN, S</creatorcontrib><creatorcontrib>KLEIN, W</creatorcontrib><title>EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts</title><title>Cardiovascular drugs and therapy</title><addtitle>Cardiovasc Drugs Ther</addtitle><description>Several authors have alluded to the possible involvement of EDRF (NO) in ischemia-induced coronary artery dilation. Alternatively, it has been suggested that opening of ATP-dependent K channels could play a key role in this context. We studied the effects of sulfonylureas and NG-nitro-L-arginine (LNNA), a specific inhibitor of endothelial NO (EDRF) synthesis, on ischemia-induced coronary vasodilation in isolated Langendorff-perfused guinea pig hearts arrested with 15 mM KCl in normal Tyrode, and isolated pig coronary arteries precontracted with 43 mM KCl. In Isolated Langerdorff-perfused guinea pig heart, when hypoxia was simulated by switching 100% O2 in the perfusate to 100% N2, coronary perfusion pressure (CPP) fell from 90 cm H2O by 45 +/- 5 cm H2O. In the presence of LNNA, a specific inhibitor of NO synthetase in endothelial cells, CPP dropped by 44 +/- 6 cm H2O (n = 6; +/- SEM, no statistically significant). On biochemical simulation of ischemia (addition of iodoacetate [IAA]), CPP dropped 40 +/- 6 cm H2O, and in experiments performed under the same conditions but in the presence of LNNA, CPP dropped by 38 +/- 5 cm H2O (n = 6; +/- SEM; not statistically significant). When ischemia was simulated metabolically by equimolar replacement of 10 mM glucose with 2-deoxyglucose (DOG), an inhibitor of glycolysis CPP decreased by 24 +/- 1 cm H2O (n = 6; +/- SEM) after 15 minutes. This fall in CPP was almost prevented by 20 microM glibenclamide, whereas in the presence of 20 microM LNNA the DOG-induced decrease in CPP was not significantly inhibited, and CPP decreased by 22 +/- 2.6 cm H2O (n = 6; +/- SEM). In isolated pig coronary artery rings, maximal tension, achieved by depolarizing the smooth muscle cells by 43 mM KCl, decreased by 37 +/- 7% upon simulated hypoxia by replacing 100% O2 with 100% N2 in the perfusate (n = 6; +/- SEM) in arteries with intact endothelium. In arteries without endothelium, maximal tension also dropped by 35 +/- 6% (not statistically significant). In the same experiments the decrease in tension could be largely inhibited in the presence of 50 microM glibenclamide. Our results clearly show that in isolated perfused guinea pig hearts, as well as in isolated pig coronary arteries, EDRF does not play a decisive role in the coronary dilatory response to hypoxia and ischemia.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cardiology. Vascular system</subject><subject>Coronary Circulation - drug effects</subject><subject>Coronary heart disease</subject><subject>Enzyme Inhibitors - therapeutic use</subject><subject>Glyburide - pharmacology</subject><subject>Guinea Pigs</subject><subject>Heart</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Myocardial Ischemia - chemically induced</subject><subject>Myocardial Ischemia - physiopathology</subject><subject>Nitric Oxide - physiology</subject><subject>Nitroarginine - therapeutic use</subject><subject>Nitrogen - pharmacology</subject><subject>Perfusion</subject><subject>Potassium Channels - physiology</subject><subject>Vasodilation - physiology</subject><issn>0920-3206</issn><issn>1573-7241</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNo9kM1KAzEUhQdRtP48gnAXbgOZyfwk7oq2KhYV6V5u_qaRNhmSGcFH8OnEN3JKS1cXzvk4h3uOskleNYw0RZkfZxMqCkpYQeuz7DylT0ppIwQ_zU5Fw0tBm0n2N7t_n4MOJoEPPWyMdtgbUCEGj_EbvjAF7dbYu-AhGRW83sp9gOQ2w6gbDS6pldk4hFuYQuoH_Q0j_DxdvoFaofdmnQC9hpffH-JdHwNZEIyt886bLXko60y0Q9o2ddGkNEQDzo_xYdezQN8ar0O0luzQUWyHMQVJ51pYGYx9usxOLK6Tudrfi2w5ny3vHsni9eHpbrogHS8aktdMltowKcuKWiEUFpWVDVrBa6VkZRktS65QUE411ZwLlIiNZGVZS2SUXWTXu9hukONqH110m_GHj_2yo3-z9zEpXNuIXrl0wPJK5HXN2T-8n4im</recordid><startdate>199807</startdate><enddate>199807</enddate><creator>GASSER, R</creator><creator>KÖPPEL, H</creator><creator>BRUSSEE, H</creator><creator>GRISOLD, M</creator><creator>HOLZMANN, S</creator><creator>KLEIN, W</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>199807</creationdate><title>EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts</title><author>GASSER, R ; KÖPPEL, H ; BRUSSEE, H ; GRISOLD, M ; HOLZMANN, S ; KLEIN, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p827-163b4de3bb450f99ca25fb7af986ccb5f30448ca9080d0d889abaa7b3446ba303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cardiology. Vascular system</topic><topic>Coronary Circulation - drug effects</topic><topic>Coronary heart disease</topic><topic>Enzyme Inhibitors - therapeutic use</topic><topic>Glyburide - pharmacology</topic><topic>Guinea Pigs</topic><topic>Heart</topic><topic>In Vitro Techniques</topic><topic>Medical sciences</topic><topic>Myocardial Ischemia - chemically induced</topic><topic>Myocardial Ischemia - physiopathology</topic><topic>Nitric Oxide - physiology</topic><topic>Nitroarginine - therapeutic use</topic><topic>Nitrogen - pharmacology</topic><topic>Perfusion</topic><topic>Potassium Channels - physiology</topic><topic>Vasodilation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GASSER, R</creatorcontrib><creatorcontrib>KÖPPEL, H</creatorcontrib><creatorcontrib>BRUSSEE, H</creatorcontrib><creatorcontrib>GRISOLD, M</creatorcontrib><creatorcontrib>HOLZMANN, S</creatorcontrib><creatorcontrib>KLEIN, W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Cardiovascular drugs and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GASSER, R</au><au>KÖPPEL, H</au><au>BRUSSEE, H</au><au>GRISOLD, M</au><au>HOLZMANN, S</au><au>KLEIN, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts</atitle><jtitle>Cardiovascular drugs and therapy</jtitle><addtitle>Cardiovasc Drugs Ther</addtitle><date>1998-07</date><risdate>1998</risdate><volume>12</volume><issue>3</issue><spage>279</spage><epage>284</epage><pages>279-284</pages><issn>0920-3206</issn><eissn>1573-7241</eissn><coden>CDTHET</coden><abstract>Several authors have alluded to the possible involvement of EDRF (NO) in ischemia-induced coronary artery dilation. Alternatively, it has been suggested that opening of ATP-dependent K channels could play a key role in this context. We studied the effects of sulfonylureas and NG-nitro-L-arginine (LNNA), a specific inhibitor of endothelial NO (EDRF) synthesis, on ischemia-induced coronary vasodilation in isolated Langendorff-perfused guinea pig hearts arrested with 15 mM KCl in normal Tyrode, and isolated pig coronary arteries precontracted with 43 mM KCl. In Isolated Langerdorff-perfused guinea pig heart, when hypoxia was simulated by switching 100% O2 in the perfusate to 100% N2, coronary perfusion pressure (CPP) fell from 90 cm H2O by 45 +/- 5 cm H2O. In the presence of LNNA, a specific inhibitor of NO synthetase in endothelial cells, CPP dropped by 44 +/- 6 cm H2O (n = 6; +/- SEM, no statistically significant). On biochemical simulation of ischemia (addition of iodoacetate [IAA]), CPP dropped 40 +/- 6 cm H2O, and in experiments performed under the same conditions but in the presence of LNNA, CPP dropped by 38 +/- 5 cm H2O (n = 6; +/- SEM; not statistically significant). When ischemia was simulated metabolically by equimolar replacement of 10 mM glucose with 2-deoxyglucose (DOG), an inhibitor of glycolysis CPP decreased by 24 +/- 1 cm H2O (n = 6; +/- SEM) after 15 minutes. This fall in CPP was almost prevented by 20 microM glibenclamide, whereas in the presence of 20 microM LNNA the DOG-induced decrease in CPP was not significantly inhibited, and CPP decreased by 22 +/- 2.6 cm H2O (n = 6; +/- SEM). In isolated pig coronary artery rings, maximal tension, achieved by depolarizing the smooth muscle cells by 43 mM KCl, decreased by 37 +/- 7% upon simulated hypoxia by replacing 100% O2 with 100% N2 in the perfusate (n = 6; +/- SEM) in arteries with intact endothelium. In arteries without endothelium, maximal tension also dropped by 35 +/- 6% (not statistically significant). In the same experiments the decrease in tension could be largely inhibited in the presence of 50 microM glibenclamide. Our results clearly show that in isolated perfused guinea pig hearts, as well as in isolated pig coronary arteries, EDRF does not play a decisive role in the coronary dilatory response to hypoxia and ischemia.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>9784907</pmid><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0920-3206
ispartof Cardiovascular drugs and therapy, 1998-07, Vol.12 (3), p.279-284
issn 0920-3206
1573-7241
language eng
recordid cdi_pubmed_primary_9784907
source Springer Link
subjects Adenosine Triphosphate - pharmacology
Animals
Biological and medical sciences
Cardiology. Vascular system
Coronary Circulation - drug effects
Coronary heart disease
Enzyme Inhibitors - therapeutic use
Glyburide - pharmacology
Guinea Pigs
Heart
In Vitro Techniques
Medical sciences
Myocardial Ischemia - chemically induced
Myocardial Ischemia - physiopathology
Nitric Oxide - physiology
Nitroarginine - therapeutic use
Nitrogen - pharmacology
Perfusion
Potassium Channels - physiology
Vasodilation - physiology
title EDRF does not mediate coronary vasodilation secondary to simulated ischemia : A study on KATP channels and Nω-nitro-L-arginine on coronary perfusion pressure in isolated Langendorff-perfused guinea-pig hearts
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A06%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=EDRF%20does%20not%20mediate%20coronary%20vasodilation%20secondary%20to%20simulated%20ischemia%20:%20A%20study%20on%20KATP%20channels%20and%20N%CF%89-nitro-L-arginine%20on%20coronary%20perfusion%20pressure%20in%20isolated%20Langendorff-perfused%20guinea-pig%20hearts&rft.jtitle=Cardiovascular%20drugs%20and%20therapy&rft.au=GASSER,%20R&rft.date=1998-07&rft.volume=12&rft.issue=3&rft.spage=279&rft.epage=284&rft.pages=279-284&rft.issn=0920-3206&rft.eissn=1573-7241&rft.coden=CDTHET&rft_id=info:doi/&rft_dat=%3Cpubmed_pasca%3E9784907%3C/pubmed_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-p827-163b4de3bb450f99ca25fb7af986ccb5f30448ca9080d0d889abaa7b3446ba303%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/9784907&rfr_iscdi=true