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Interleukin-8: an autocrine/paracrine growth factor for human hematopoietic progenitors acting in synergy with colony stimulating factor-1 to promote monocyte-macrophage growth and differentiation

We previously reported that alteration of the function of heterotrimeric G i2 proteins altered proliferation of murine macrophages in response to colony stimulating factor-1 (CSF-1). Here we show that a G i2 agonist, C-X-C chemokine interleukin-8 (IL-8), regulates monocyte-macrophage growth and diff...

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Bibliographic Details
Published in:Experimental hematology 1999, Vol.27 (1), p.28-36
Main Authors: Corre, Isabelle, Pineau, Danielle, Hermouet, Sylvie
Format: Article
Language:English
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Summary:We previously reported that alteration of the function of heterotrimeric G i2 proteins altered proliferation of murine macrophages in response to colony stimulating factor-1 (CSF-1). Here we show that a G i2 agonist, C-X-C chemokine interleukin-8 (IL-8), regulates monocyte-macrophage growth and differentiation. In the absence of serum, IL-8 (10 ng/mL) synergized with CSF-1 to stimulate murine monocyte-macrophage proliferation, enhanced proliferation of purified human CD34 + cells and increased the number and size of CSF-1–induced monocyte-macrophage colonies formed by purified CD34 + cells in semisolid medium. Next, as both CD34 + cells and monocyte-macrophages can produce IL-8, we used an anti-human IL-8 antibody to block an eventual activation of IL-8 receptors by autocrine IL-8. Preincubation with anti-human IL-8 antibody (20–40 μg/mL) inhibited the proliferation as well as the monocyte-macrophage colony clonogenicity of purified human CD34 + cells. Hence, in addition to being a powerful neutrophil chemoattractant, IL-8 also acts as an autocrine/paracrine growth factor for human hematopoietic progenitors, promoting the growth and differentiation of cells of monocytic lineage.
ISSN:0301-472X
1873-2399
DOI:10.1016/S0301-472X(98)00032-0