Epigallocatechin Gallate: pH-Dependent Redox Properties and Effect on Respiration, Photosynthesis, and Cell Death in Pea Plants

• Published in: Biochemistry (Moscow) 2023-02, Vol.88 (2), p.211-220
• Main Authors: Kiselevsky, Dmitry B., Samuilova, Olga V., Samuilov, Vitaly D.
• Format: Article
• Language: English
• Subjects: Analysis; Apoptosis; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Catechin; Catechin - pharmacology; Cell Death; Electron transfer; Electron Transport; Electron transport chain; Epidermis; Epigallocatechin gallate; Green tea; Growth; Guard cells; Hydrogen peroxide; Hydrogen Peroxide - metabolism; Hydrogen-Ion Concentration; Identification and classification; Iodides; Leaves; Life Sciences; Membrane permeability; Membrane potential; Microbiology; Mitochondria; Nicotinamide adenine dinucleotide; Oxygen; Peas; Peroxidase; pH effects; Photosynthesis; Photosystem II; Pisum sativum - metabolism; Plant cells; Properties; Propidium iodide; Reactive oxygen species; Reactive Oxygen Species - metabolism; Redox properties; Respiration
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297923020050

In vitro redox properties of the green tea component epigallocatechin gallate (EGCG) and its effect on pea plant cells were investigated. EGCG was found to exhibit both pro- and antioxidant properties. In solutions, EGCG was oxidized by oxygen at physiological (slightly alkaline) pH values with the generation of O 2 –• and H 2 O 2 , the reaction being slowed down by a decrease in the medium pH. On the other hand, EGCG functioned as an electron donor for peroxidase, resulting in the H 2 O 2 utilization. EGCG suppressed respiration, reduced mitochondrial transmembrane potential difference and inhibited electron transfer in the photosynthetic electron transport chain in pea leaf cells (leaf cuttings and epidermis). Among components of the photosynthetic redox chain, Photosystem II was the least sensitive to the EGCG action. In the epidermis, EGCG reduced the rate of reactive oxygen species formation that was induced by NADH. EGCG at the concentrations from 10 μM to 1 mM suppressed the KCN-induced death of guard cells in the epidermis, which was determined from the destruction of cell nuclei. EGCG at a concentration of 10 mM disrupted the barrier function of the guard cell plasma membrane, increasing its permeability to propidium iodide.