Does Endometriosis Impact the Composition of Follicular Fluid in IL6 and AMH? A Case-Control Study

• Published in: Journal of clinical medicine 2023-02, Vol.12 (5), p.1829
• Main Authors: Kacem-Berjeb, Khadija, Braham, Marouen, Massoud, Cyrine Ben, Hannachi, Hela, Hamdoun, Manel, Chtourou, Sana, Debbabi, Linda, Bouyahia, Maha, Fadhlaoui, Anis, Zhioua, Fethi, Feki, Anis, Chakroun, Nozha, Bahri, Olfa
• Format: Article
• Language: English
• Subjects: Age; Analysis; Anti-Müllerian hormone; Care and treatment; Clinical medicine; Cytokines; Diagnosis; Embryos; Endometriosis; Fertility; Infertility; Interleukin-6; Ovaries; Statistical analysis; Ultrasonic imaging; Womens health
• ISSN: 2077-0383; 2077-0383
• DOI: 10.3390/jcm12051829

The aim of this study was to compare follicular liquid levels of IL6 and AMH in women with and without endometriosis and to evaluate their potential impact on ICSI outcomes. It is a prospective case-control study conducted on 25 women with proven endometriosis and 50 patients diagnosed with other causes of infertility. All these patients were candidates for ICSI cycles. Their follicular fluid was collected at the time of oocyte retrieval and used to evaluate IL-6 and AMH titers by electro-chemiluminescent immunoassay (Cobas e411-Roche). The IL-6 levels in follicular fluid were higher in the endometriosis group than in the control group (152.3 vs. 19.9 pg/mL; = 0.02). The median level for AMH was 2.2 ± 1.88 ng/mL with no statistical difference between the two groups (2.2 vs. 2.7 ng/mL, = 0.41). No significant correlation between the follicular IL6 and AMH levels was observed. The oocyte quality seems to be preserved in patients with endometriosis with the adequate response to ovarian stimulation. High levels of follicular IL6 are in accordance with the inflammatory phenomenon of the disease; however, this increase has no impact on ICSI outcomes.