The functional views on response of host rabbit post coronavirus vaccination via ACE2 PET

• Published in: American journal of nuclear medicine and molecular imaging 2023-01, Vol.13 (1), p.43-50
• Main Authors: Li, Xiao, Li, Jie, Zhou, Pan, Li, Danni, Wang, Mingxin, Tong, Qianqian, Chen, Jian, Zuo, Changjing, Zhang, Lan, Li, Rou
• Format: Article
• Language: English
• Subjects: Original
• ISSN: 2160-8407; 2160-8407

Molecular imaging can dynamically and quantitatively record the biochemical changes in a systemic view. In this research, SARS-CoV-2 pseudovirus was intramuscularly injected to simulate the vaccination with inactivated virus. New Zealand white rabbits were evaluated with F-FDG PET for inflammation and Ga-cyc-DX600 PET for ACE2 fluctuation, which were performed before and at 3, 7 and 14 days post injection (d P.I.); furthermore, one rabbit was vaccinated with two cycles with interval of 14 days for a longer period evaluation. Different with the vaccination-induced inflammatory response that was random and individual, ACE2 regulation was systemic and organ-specific: the liver and spleen were of a moderate decrease post injection but rebound at 14 d P.I., while there were a downward trend in heart, testis and bone marrow; besides, similar pattern of ACE2 regulation were recorded after the second injection with a relatively greater volatility. In conclusion, ACE2 PET gave a more comprehensive view on host response post vaccination, hold substantial promise in continuous monitoring of coronavirus vaccine administration and effectiveness.