Effects of D-penicillamine on growth and cell cycle kinetics of cultured rabbit articular chondrocytes

The long-acting antirheumatic drug D-penicillamine was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose-related between 5 X 10(-4) M and 5 X 10(-3) M and was time-dependent for a given dose. Flow cytometric analysis showed that dr...

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Bibliographic Details
Published in:Annals of the rheumatic diseases 1984-04, Vol.43 (2), p.333-338
Main Authors: Jaffray, P, Ronot, X, Adolphe, M, Fontagne, J, Lechat, P
Format: Article
Language:English
Subjects:
Animals
Cartilage, Articular - analysis
Cartilage, Articular - cytology
Cartilage, Articular - drug effects
Cell Cycle - drug effects
Cell Division - drug effects
Cells, Cultured
Clone Cells - drug effects
DNA - analysis
Dose-Response Relationship, Drug
Flow Cytometry
Penicillamine - pharmacology
Rabbits
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Summary:The long-acting antirheumatic drug D-penicillamine was found to inhibit the growth of asynchronous cultures of rabbit articular chondrocytes. This inhibitory effect was dose-related between 5 X 10(-4) M and 5 X 10(-3) M and was time-dependent for a given dose. Flow cytometric analysis showed that drug exposure led to a slowdown in cell cycle progression. This was manifested as a decrease in the number of cells in S phase, due especially to an accumulation of cells in G0 G1 and also to a slight cessation of cell transit through G2 M. Recovery experiments showed that the effect is transitory and reversible. It is suggested that the articular chondrocyte is a target cell for D-penicillamine and that these cells have a D-penicillamine sensitive restriction point in the G0 G1 phase of the cell cycle and to a less extent in the G2 M phase.
ISSN:0003-4967
1468-2060
DOI:10.1136/ard.43.2.333