Effects of polyphenols in non-centrifugal cane sugar on saliva secretion: in vitro and in vivo experiments and a randomized controlled trial

This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Biochemistry and Nutrition 2023, Vol.72(2), pp.171-182
Main Authors: Supriya, Shakya, Ushikoshi-Nakayama, Ryoko, Yamazaki, Tomoe, Omagari, Daisuke, Aota, Keiko, Inoue, Hiroko, Matsumoto, Naoyuki, Saito, Ichiro
Format: Article
Language:English
Subjects:
Acetylcholine receptors (muscarinic)
Blood flow
Calcium (intracellular)
Carbachol
Clinical trials
Endothelial cells
Hydrogen peroxide
In vivo methods and tests
Intracellular
Nitric oxide
Nitric-oxide synthase
non-centrifugal cane sugar
Original
Phosphorylation
Polyphenols
randomized controlled trial
Reactive oxygen species
Saliva
Salivary gland
Salivary glands
salivation
Saponarin
Secretion
Stimulation
Submandibular gland
Sugar
Translocation
Vasodilation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study examined the bioactivities and mechanisms of the non-centrifugal cane sugar polyphenols saponarin, schaftoside, and isoschaftoside in the salivary gland and their effects on salivation. In acute isolated C57BL/6N mouse submandibular gland cells, these polyphenols led to a higher increase in intracellular calcium after stimulation with the muscarinic agonist carbachol. Stimulation of these cells with polyphenols enhanced ATP production, aquaporin-5 translocation to the plasma membrane and eliminated intracellular reactive oxygen species generated by H2O2. In addition, phosphorylation of endothelial nitric oxide synthase and increased nitric oxide production in vascular endothelial cells were observed. In vivo administration of these polyphenols to C57BL/6N male mice resulted in significantly increased blood flow (saponarin, p = 0.040; isoschaftoside, p = 0.010) and salivation (saponarin, p = 0.031). A randomized controlled trial showed that intake of non-centrifugal cane sugar significantly increased saliva secretion compared with placebo (p = 0.003). These data suggest that non-centrifugal cane sugar polyphenols affect several pathways that support salivation and increase saliva secretion by enhancing vasodilation. Hence, non-centrifugal cane sugar polyphenols can be expected to maintain saliva secretion and improve reduced saliva flow.
ISSN:0912-0009
1880-5086
DOI:10.3164/jcbn.22-114