Effects of dexamethasone on the growth of cultured rabbit articular chondrocytes:relation with the nuclear glucocorticoid-receptor complex

This study reports that dexamethasone at a high dose (10(-4) mol/l) induced slowing of the in vitro proliferation of rabbit articular chondrocytes in both monolayer and clonal culture. This effect is consistent with an inhibition of DNA and RNA synthesis and was characterised by an accumulation of c...

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Bibliographic Details
Published in:Annals of the rheumatic diseases 1987-02, Vol.46 (2), p.146-152
Main Authors: Hainque, B, Dominice, J, Jaffray, P, Ronot, X, Adolphe, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Bones, joints and connective tissue. Antiinflammatory agents
Cartilage, Articular - cytology
Cartilage, Articular - drug effects
Cartilage, Articular - metabolism
Cell Division - drug effects
Cell Nucleus - drug effects
Cells, Cultured
Dexamethasone - pharmacology
DNA - biosynthesis
Flow Cytometry
Macromolecular Substances
Medical sciences
Pharmacology. Drug treatments
Rabbits
Receptors, Glucocorticoid - drug effects
RNA - biosynthesis
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Summary:This study reports that dexamethasone at a high dose (10(-4) mol/l) induced slowing of the in vitro proliferation of rabbit articular chondrocytes in both monolayer and clonal culture. This effect is consistent with an inhibition of DNA and RNA synthesis and was characterised by an accumulation of cells in the G0G1 phase of the cell cycle, as shown by flow cytometric analysis. Therefore we determined the extent of nuclear localisation of dexamethasone-receptor complexes. The results showed a discrepancy between 50% growth inhibitory dose (10(-4) mol/l) and the apparent affinity, KD (1.4 (SD 0.2) X 10(-9) mol/l). Thus the growth inhibition of rabbit articular chondrocytes by dexamethasone did not seem to be related exclusively to an interaction with the glucocorticoid-receptor complexes.
ISSN:0003-4967
1468-2060
DOI:10.1136/ard.46.2.146