Systematic Comparison of Commercial Hydrogels Revealed That a Synergy of Laminin and Strain-Stiffening Promotes Directed Migration of Neural Cells

Hydrogels have shown potential in replacing damaged nerve tissue, but the ideal hydrogel is yet to be found. In this study, various commercially available hydrogels were compared. Schwann cells, fibroblasts, and dorsal root ganglia neurons were seeded on the hydrogels, and their morphology, viabilit...

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Published in:ACS applied materials & interfaces 2023-03, Vol.15 (10), p.12678-12695
Main Authors: Millesi, Flavia, Mero, Sascha, Semmler, Lorenz, Rad, Anda, Stadlmayr, Sarah, Borger, Anton, Supper, Paul, Haertinger, Maximilian, Ploszczanski, Leon, Windberger, Ursula, Weiss, Tamara, Naghilou, Aida, Radtke, Christine
Format: Article
Language:English
Subjects:
Biological and Medical Applications of Materials and Interfaces
Cell Movement
Hydrogels - chemistry
Hydrogels - pharmacology
Laminin - pharmacology
Neurons
Schwann Cells
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Summary:Hydrogels have shown potential in replacing damaged nerve tissue, but the ideal hydrogel is yet to be found. In this study, various commercially available hydrogels were compared. Schwann cells, fibroblasts, and dorsal root ganglia neurons were seeded on the hydrogels, and their morphology, viability, proliferation, and migration were examined. Additionally, detailed analyses of the gels’ rheological properties and topography were conducted. Our results demonstrate vast differences on cell elongation and directed migration on the hydrogels. Laminin was identified as the driver behind cell elongation and in combination with a porous, fibrous, and strain-stiffening matrix structure responsible for oriented cell motility. This study improves our understanding of cell–matrix interactions and thereby facilitates tailored fabrication of hydrogels in the future.
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.2c20040