Exploring structural features and potential lipid interactions of Pseudomonas aeruginosa type three secretion effector PemB by spectroscopic and calorimetric experiments

Type Three Secretion System (T3SS) is a sophisticated nano‐scale weapon utilized by several gram negative bacteria under stringent spatio‐temporal regulation to manipulate and evade host immune systems in order to cause infection. To the best of our knowledge, this present study is the first report...

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Bibliographic Details
Published in:Protein science 2023-04, Vol.32 (4), p.e4627-n/a
Main Authors: Choudhury, Arkaprabha, Saha, Saumen, Maiti, Nakul Chandra, Datta, Saumen
Format: Article
Language:English
Subjects:
Aqueous solutions
Bacterial Proteins - chemistry
effector
Full‐length Paper
Full‐length Papers
Gram-negative bacteria
Heat measurement
Immune system
Lipids
Oligomerization
Phosphoinositides
Phylogeny
Pseudomonas aeruginosa
Pseudomonas aeruginosa - chemistry
Raman spectroscopy
Secretome
Spectroscopy
Type III Secretion Systems - chemistry
type three secretion system
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Summary:Type Three Secretion System (T3SS) is a sophisticated nano‐scale weapon utilized by several gram negative bacteria under stringent spatio‐temporal regulation to manipulate and evade host immune systems in order to cause infection. To the best of our knowledge, this present study is the first report where we embark upon characterizing inherent features of native type three secretion effector protein PemB through biophysical techniques. Herein, first, we demonstrate binding affinity of PemB for phosphoinositides through isothermal calorimetric titrations. Second, we shed light on its strong homo‐oligomerization propensity in aqueous solution through multiple biophysical methods. Third, we also employ several spectroscopic techniques to delineate its disordered and helical conformation. Lastly, we perform a phylogenetic analysis of this new effector to elucidate evolutionary relationship with other organisms. Taken together, our results shall surely contribute to our existing knowledge of Pseudomonas aeruginosa secretome.
ISSN:0961-8368
1469-896X
DOI:10.1002/pro.4627