Detecting Embryo Developmental Potential by Single Blastomere RNA-Seq

Recent advances in preimplantation embryo diagnostics enable a wide range of applications using single cell biopsy and molecular-based selection techniques without compromising embryo production. This study was conducted to develop a single cell embryo biopsy technique and gene expression analysis m...

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Bibliographic Details
Published in:Genes 2023-02, Vol.14 (3), p.569
Main Authors: Nõmm, Monika, Ivask, Marilin, Pärn, Pille, Reimann, Ene, Kõks, Sulev, Jaakma, Ülle
Format: Article
Language:English
Subjects:
Animals
Biopsy
Blastocyst
Blastocysts
Blastomeres
Cattle
Developmental stages
Embryonic development
Embryonic Development - genetics
Embryos
Female
Fertilization in Vitro - methods
Gene expression
Genetic aspects
In vitro fertilization
Mineral oils
Mitochondria
Physiological aspects
Pregnancy
Preimplantation Diagnosis - methods
RNA sequencing
RNA, Messenger
RNA-Seq
Software
Sperm
Statistical analysis
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Summary:Recent advances in preimplantation embryo diagnostics enable a wide range of applications using single cell biopsy and molecular-based selection techniques without compromising embryo production. This study was conducted to develop a single cell embryo biopsy technique and gene expression analysis method with a very low input volume to ensure normal embryo development and to see if there are differences in gene expression profiles between day-5 biopsied bovine embryos that developed into blastocysts and embryos arrested at morula stage. Out of the 65 biopsied morulae, 32 developed to blastocysts (49.2%). Out of the 13,580 successfully annotated genes, 1204 showed a difference in mRNA expression level. Out of these, 155 genes were expressed in embryos developing to blastocysts. The pathway enrichment analysis revealed significant enrichment in "organelle biogenesis and maintenance", "mRNA splicing" and "mitochondrial translation" pathways. These findings suggest principal differences in gene expression patterns and functional networks of embryos able to reach the blastocyst stage compared to embryos arrested in development. Our preliminary data suggest that single blastomere biopsy and selected gene expression profiles at morula stage could offer additional possibilities for early preimplantation embryo selection before transfer.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes14030569