Characterization and Vaccine Potential of Outer Membrane Vesicles from Photobacterium damselae subsp. piscicida

subsp. ( ) is a Gram-negative fish pathogen with worldwide distribution and broad host specificity that causes heavy economic losses in aquaculture. Although was first identified more than 50 years ago, its pathogenicity mechanisms are not completely understood. In this work, we report that secretes...

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Published in:International journal of molecular sciences 2023-03, Vol.24 (6), p.5138
Main Authors: Teixeira, Alexandra, Loureiro, Inês, Lisboa, Johnny, Oliveira, Pedro N, Azevedo, Jorge E, Dos Santos, Nuno M S, do Vale, Ana
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Antigens
Antimicrobial agents
Antimicrobial peptides
Aquaculture
Bactericidal activity
Bass
Economic impact
Fish Diseases
Gram-Negative Bacterial Infections - prevention & control
Gram-Negative Bacterial Infections - veterinary
Host specificity
Infections
Membrane vesicles
Membranes
Pathogenicity
Pathogens
Peptides
Photobacterium
Photobacterium damselae
Proteins
Sea bass
Vaccines
Vesicles
Virulence
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Summary:subsp. ( ) is a Gram-negative fish pathogen with worldwide distribution and broad host specificity that causes heavy economic losses in aquaculture. Although was first identified more than 50 years ago, its pathogenicity mechanisms are not completely understood. In this work, we report that secretes large amounts of outer membrane vesicles (OMVs) when cultured in vitro and during in vivo infection. These OMVs were morphologically characterized and the most abundant vesicle-associated proteins were identified. We also demonstrate that OMVs protect cells from the bactericidal activity of fish antimicrobial peptides, suggesting that secretion of OMVs is part of the strategy used by to evade host defense mechanisms. Importantly, the vaccination of sea bass ( ) with adjuvant-free crude OMVs induced the production of anti- antibodies and resulted in partial protection against infection. These findings reveal new aspects of biology and may provide a basis for developing new vaccines against this pathogen.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24065138