Carbon dots as a versatile tool to monitor insulin aggregation

The possibility to monitor peptide and protein aggregation is of paramount importance in the so-called conformational diseases, as the understanding of many physiological pathways, as well as pathological processes involved in the development of such diseases, depends very much on the actual possibi...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2023-04, Vol.415 (10), p.1829-1840
Main Authors: Zingale, Gabriele Antonio, Distefano, Alessia, Pandino, Irene, Tuccitto, Nunzio, Oliveri, Valentina, Gaeta, Massimiliano, D’Urso, Alessandro, Arcoria, Alfio, Grasso, Giuseppe
Format: Article
Language:English
Subjects:
Agglomeration
Analysis
Analytical Chemistry
Biochemistry
Biomolecules
Carbon
Carbon - chemistry
Carbon dots
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Circular Dichroism
Dichroism
Experimental methods
Fluorescence
Fluorescent Dyes - chemistry
Food Science
Insulin
Insulin - chemistry
Laboratory Medicine
Mechanical properties
Monitoring methods
Monitoring/Environmental Analysis
Optical properties
Protein Aggregates
Protein binding
Protein interaction
Proteins
Quantum dots
Quantum Dots - chemistry
Research Paper
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Summary:The possibility to monitor peptide and protein aggregation is of paramount importance in the so-called conformational diseases, as the understanding of many physiological pathways, as well as pathological processes involved in the development of such diseases, depends very much on the actual possibility to monitor biomolecule oligomeric distribution and aggregation. In this work, we report a novel experimental method to monitor protein aggregation, based on the change of the fluorescent properties of carbon dots upon protein binding. The results obtained in the case of insulin with this newly proposed experimental approach are compared with those obtained with other common experimental techniques normally used for the same purpose (circular dichroism, DLS, PICUP and ThT fluorescence). The greatest advantage of the hereby presented methodology over all the other experimental methods considered is the possibility to monitor the initial stages of insulin aggregation under the different experimental conditions sampled and the absence of possible disturbances and/or molecular probes during the aggregation process. Graphical abstract
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-023-04585-y