PRMT6-CDC20 facilitates glioblastoma progression via the degradation of CDKN1B

• Published in: Oncogene 2023-03, Vol.42 (14), p.1088-1100
• Main Authors: Wang, Ji, Xiao, Zongyu, Li, Peng, Wu, Chunwang, Li, Yan, Wang, Qing, Chen, Yanming, Zhou, Honglong, Li, Zhi, Wang, Zhaotao, Lan, Qing, Wang, Yezhong
• Format: Article
• Language: English
• Subjects: 13/109; 13/89; 38; 631/67/1922; 631/67/2324; 82/81; 96/1; Apoptosis; Brain cancer; Brain tumors; Cdc20 Proteins - genetics; Cdc20 Proteins - metabolism; Cell Biology; Cell cycle; Cell Cycle Proteins - metabolism; Cell growth; Cell proliferation; Cyclin-Dependent Kinase Inhibitor p27 - metabolism; Degradation; Epigenetics; G1 phase; Glioblastoma; Glioblastoma - genetics; Glioma; Histones; Histones - genetics; Histones - metabolism; Human Genetics; Humans; Internal Medicine; Malignancy; Medicine; Medicine & Public Health; Methylation; Nuclear Proteins - metabolism; Oncology; Protein arginine methyltransferase; Protein-Arginine N-Methyltransferases - genetics; Protein-Arginine N-Methyltransferases - metabolism
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/s41388-023-02624-7

PRMT6, a type I arginine methyltransferase, di-methylates the arginine residues of both histones and non-histones asymmetrically. Increasing evidence indicates that PRMT6 plays a tumor mediator involved in human malignancies. Here, we aim to uncover the essential role and underlying mechanisms of PRMT6 in promoting glioblastoma (GBM) proliferation. Investigation of PRMT6 expression in glioma tissues demonstrated that PRMT6 is overexpressed, and elevated expression of PRMT6 is negatively correlated with poor prognosis in glioma/GBM patients. Silencing PRMT6 inhibited GBM cell proliferation and induced cell cycle arrest at the G0/G1 phase, while overexpressing PRMT6 had opposite results. Further, we found that PRMT6 attenuates the protein stability of CDKN1B by promoting its degradation. Subsequent mechanistic investigations showed that PRMT6 maintains the transcription of CDC20 by activating histone methylation mark (H3R2me2a), and CDC20 interacts with and destabilizes CDKN1B. Rescue experimental results confirmed that PRMT6 promotes the ubiquitinated degradation of CDKN1B and cell proliferation via CDC20. We also verified that the PRMT6 inhibitor (EPZ020411) could attenuate the proliferative effect of GBM cells. Our findings illustrate that PRMT6, an epigenetic mediator, promotes CDC20 transcription via H3R2me2a to mediate the degradation of CDKN1B to facilitate GBM progression. Targeting PRMT6-CDC20-CDKN1B axis might be a promising therapeutic strategy for GBM.