The histone demethylase Kdm3 prevents auto-immune piRNAs production in Drosophila

Genome integrity of the animal germline is protected from transposable element activity by PIWI-interacting RNAs (piRNAs). While piRNA biogenesis is intensively explored, little is known about the genetical determination of piRNA clusters, the genomic sources of piRNAs. Using a bimodal epigenetic st...

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Bibliographic Details
Published in:Science advances 2023-04, Vol.9 (14), p.eade3872
Main Authors: Casier, Karine, Autaa, Julie, Gueguen, Nathalie, Delmarre, Valérie, Marie, Pauline P, Ronsseray, Stéphane, Carré, Clément, Brasset, Emilie, Teysset, Laure, Boivin, Antoine
Format: Article
Language:English
Subjects:
Animals
Biomedicine and Life Sciences
DNA Transposable Elements - genetics
Drosophila - genetics
Drosophila - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Genetics
Life Sciences
Piwi-Interacting RNA
RNA, Small Interfering - genetics
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Summary:Genome integrity of the animal germline is protected from transposable element activity by PIWI-interacting RNAs (piRNAs). While piRNA biogenesis is intensively explored, little is known about the genetical determination of piRNA clusters, the genomic sources of piRNAs. Using a bimodal epigenetic state piRNA cluster ( ), we identified the histone demethylase Kdm3 as being able to prevent a cryptic piRNA production. In the absence of Kdm3, dozens of coding gene-containing regions become genuine germline dual-strand piRNA clusters. Eggs laid by mutant females show developmental defects phenocopying loss of function of genes embedded into the additional piRNA clusters, suggesting an inheritance of functional ovarian "auto-immune" piRNAs. Antagonizing piRNA cluster determination through chromatin modifications appears crucial to prevent auto-immune genic piRNAs production.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.ade3872