Single-cell transcriptomic heterogeneity between conduit and resistance mesenteric arteries in rats

The endothelium contains morphologically similar cells throughout the vasculature, but individual cells along the length of a single vascular tree or in different regional circulations function dissimilarly. When observations made in large arteries are extrapolated to explain the function of endothe...

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Bibliographic Details
Published in:Physiological genomics 2023-04, Vol.55 (4), p.179-193
Main Authors: Anderson, Jacob R, Morin, Emily E, Brayer, Kathryn J, Salbato, Sophia, Gonzalez Bosc, Laura V, Kanagy, Nancy L, Naik, Jay S
Format: Article
Language:English
Subjects:
Animals
Endothelial Cells - metabolism
Endothelium, Vascular - metabolism
Gene Expression Profiling
Mesenteric Arteries
Muscle, Smooth, Vascular - metabolism
Rats
Rats, Sprague-Dawley
Transcriptome - genetics
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Summary:The endothelium contains morphologically similar cells throughout the vasculature, but individual cells along the length of a single vascular tree or in different regional circulations function dissimilarly. When observations made in large arteries are extrapolated to explain the function of endothelial cells (ECs) in the resistance vasculature, only a fraction of these observations are consistent between artery sizes. To what extent endothelial (EC) and vascular smooth muscle cells (VSMCs) from different arteriolar segments of the same tissue differ phenotypically at the single-cell level remains unknown. Therefore, single-cell RNA-seq (10x Genomics) was performed using a 10X Genomics Chromium system. Cells were enzymatically digested from large (>300 µm) and small (
ISSN:1094-8341
1531-2267
DOI:10.1152/physiolgenomics.00126.2022