3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy

Summary Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2023-02, Vol.289 (2), p.128-133
Main Authors: Verrier, Nicolas, Taddese, Asemare Mengistie, Abbessi, Riadh, Debailleul, Matthieu, Haeberlé, Olivier
Format: Article
Language:English
Subjects:
Diffraction
Engineering Sciences
holography
Holography - methods
Illumination
Image reconstruction
Interference
Microscopy
Microscopy, Interference - methods
Microscopy, Polarization
Optics
Photonic
Polarization
Refractivity
Refractometry
Sensor arrays
Technical Notes
Tomography
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Summary:Summary Tomographic diffraction microscopy (TDM) is a generalisation of digital holographic microscopy (DHM), for which the illumination angle onto the sample is fully controlled, which has become a tool of choice for 3D, high‐resolution imaging of unlabelled samples. TDM makes it possible to obtain the optical field in both amplitude and phase for each illumination angle. Proper information reallocation eventually allows for 3D reconstruction of the complex refractive index map. On the other hand, polarisation array sensors (PAS) paves new way for TDM, as vectorial information assessment about the investigated sample. In this contribution, we show an alternative use of this polarisation information based on the phase sensitive nature of TDM. Here, we demonstrated that TDM coupled with PAS can lead to a 3D differential interference contrast (DIC) microscope with almost no experimental configuration modification.
ISSN:0022-2720
1365-2818
DOI:10.1111/jmi.13160