Cortical microtubules contribute to division plane positioning during telophase in maize

Abstract Cell divisions are accurately positioned to generate cells of the correct size and shape. In plant cells, the new cell wall is built in the middle of the cell by vesicles trafficked along an antiparallel microtubule and a microfilament array called the phragmoplast. The phragmoplast expands...

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Bibliographic Details
Published in:The Plant cell 2023-04, Vol.35 (5), p.1496-1512
Main Authors: Bellinger, Marschal A, Uyehara, Aimee N, Allsman, Lindy, Martinez, Pablo, McCarthy, Michael C, Rasmussen, Carolyn G
Format: Article
Language:English
Subjects:
Arabidopsis
Cytokinesis
Microtubules - metabolism
Mitosis
Telophase
Zea mays - genetics
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Summary:Abstract Cell divisions are accurately positioned to generate cells of the correct size and shape. In plant cells, the new cell wall is built in the middle of the cell by vesicles trafficked along an antiparallel microtubule and a microfilament array called the phragmoplast. The phragmoplast expands toward a specific location at the cell cortex called the division site, but how it accurately reaches the division site is unclear. We observed microtubule arrays that accumulate at the cell cortex during the telophase transition in maize (Zea mays) leaf epidermal cells. Before the phragmoplast reaches the cell cortex, these cortical-telophase microtubules transiently interact with the division site. Increased microtubule plus end capture and pausing occur when microtubules contact the division site-localized protein TANGLED1 or other closely associated proteins. Microtubule capture and pausing align the cortical microtubules perpendicular to the division site during telophase. Once the phragmoplast reaches the cell cortex, cortical-telophase microtubules are incorporated into the phragmoplast primarily by parallel bundling. The addition of microtubules into the phragmoplast promotes fine-tuning of the positioning at the division site. Our hypothesis is that division site-localized proteins such as TANGLED1 organize cortical microtubules during telophase to mediate phragmoplast positioning at the final division plane. Microtubules accumulate at the cell cortex during telophase and interact with the plant division machinery to direct its movement towards the division site.
ISSN:1040-4651
1532-298X
DOI:10.1093/plcell/koad033