Pharmacodynamic Material Basis and Potential Mechanism Study of Spatholobi Caulis in Reversing Osteoporosis

Objective. To elucidate the mechanism of Spatholobi Caulis (SC) in treating osteoporosis (OP) integrated zebrafish model and bioinformatics. Methods. Skeleton staining coupled with image quantification was performed to evaluate the effects of SC on skeleton mineralization area (SSA) and total optica...

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Published in:Evidence-based complementary and alternative medicine 2023, Vol.2023 (1), p.3071147-3071147
Main Authors: Xiao, Jianpeng, Shang, Wei, Zhao, Zhiming, Jiang, Jun, Chen, Jianping, Cai, Hui, He, Jinjin, Cai, Zhihui, Zhao, Zihan
Format: Article
Language:English
Subjects:
AKT1 protein
Bioactive compounds
Bioinformatics
Chinese medicine
Disease
Emodin
Fractures
Gelatinase B
Genomes
Glycosylation
Interleukin 6
Licochalcone A
Ligands
Locomotor activity
Mineralization
Optical density
Osteoporosis
p53 Protein
Pharmacodynamics
Pharmacology
Proteins
Signal transduction
Therapeutic targets
Traditional Chinese medicine
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Summary:Objective. To elucidate the mechanism of Spatholobi Caulis (SC) in treating osteoporosis (OP) integrated zebrafish model and bioinformatics. Methods. Skeleton staining coupled with image quantification was performed to evaluate the effects of SC on skeleton mineralization area (SSA) and total optical density (TOD). Zebrafish locomotor activity was monitored using the EthoVision XT. Bioactive compounds of SC and their corresponding protein targets were acquired from Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Potential therapeutic targets for OP were summarized through retrieving 5 databases, and then, the overlapping genes between SC and OP were acquired. The core genes were selected by CytoHubba. Subsequently, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) functional analysis of the intersection target genes were carried out by R software. Finally, the molecular docking simulation was manipulated between the ingredients and the hub genes. Results. Compared with the model group, SC significantly increased the SSA and TOD at 10 mg/mL and improved the locomotor activity in a dose-dependent manner (p
ISSN:1741-427X
1741-4288
DOI:10.1155/2023/3071147