Immunohistochemical Detection of Synuclein Pathology in Skin in Idiopathic Rapid Eye Movement Sleep Behavior Disorder and Parkinsonism

• Published in: Movement disorders 2021-04, Vol.36 (4), p.895-904
• Main Authors: Al‐Qassabi, Ahmed, Tsao, Tsu‐Shuen, Racolta, Adriana, Kremer, Thomas, Cañamero, Marta, Belousov, Anton, Santana, Madison A., Beck, Rachel C., Zhang, Hongjun, Meridew, Jeffrey, Pugh, Judith, Lian, Fangru, Robida, Mark D., Ritter, Mirko, Czech, Christian, Beach, Thomas G., Pestic‐Dragovich, Lidija, Taylor, Kirsten I., Zago, Wagner, Tang, Lei, Dziadek, Sebastian, Postuma, Ronald B.
• Format: Article
• Language: English
• Subjects: alpha-Synuclein; Automation; Autopsy; Basal ganglia; Behavior disorders; Biopsy; Brain diseases; Central nervous system diseases; Diagnosis; Eye movements; Humans; Immunohistochemistry; Lewy bodies; Lewy Body Disease; Movement disorders; Nervous system; Neurodegenerative diseases; Parkinson Disease; Parkinson's disease; Pathology; PGP9.5; Protein gene product 9.5; RBD; REM sleep; REM Sleep Behavior Disorder; Serine; Skin; skin biopsy; Sleep; Sleep disorders; Synuclein; UCHL1
• ISSN: 0885-3185; 1531-8257
• DOI: 10.1002/mds.28399

ABSTRACT Background Recent studies reported abnormal alpha‐synuclein deposition in biopsy‐accessible sites of the peripheral nervous system in Parkinson's disease (PD). This has considerable implications for clinical diagnosis. Moreover, if deposition occurs early, it may enable tissue diagnosis of prodromal PD. Objective The aim of this study was to develop and test an automated bright‐field immunohistochemical assay of cutaneous pathological alpha‐synuclein deposition in patients with idiopathic rapid eye movement sleep behavior disorder, PD, and atypical parkinsonism and in control subjects. Methods For assay development, postmortem skin biopsies were taken from 28 patients with autopsy‐confirmed Lewy body disease and 23 control subjects. Biopsies were stained for pathological alpha‐synuclein in automated stainers using a novel dual‐immunohistochemical assay for serine 129‐phosphorylated alpha‐synuclein and pan‐neuronal marker protein gene product 9.5. After validation, single 3‐mm punch skin biopsies were taken from the cervical 8 paravertebral area from 79 subjects (28 idiopathic rapid eye movement sleep behavior disorder, 20 PD, 10 atypical parkinsonism, and 21 control subjects). Raters blinded to clinical diagnosis assessed the biopsies. Results The immunohistochemistry assay differentiated alpha‐synuclein pathology from nonpathological‐appearing alpha‐synuclein using combined phosphatase and protease treatments. Among autopsy samples, 26 of 28 Lewy body samples and none of the 23 controls were positive. Among living subjects, punch biopsies were positive in 23 (82%) subjects with idiopathic rapid eye movement sleep behavior disorder, 14 (70%) subjects with PD, 2 (20%) subjects with atypical parkinsonism, and none (0%) of the control subjects. After a 3‐year follow‐up, eight idiopathic rapid eye movement sleep behavior disorder subjects phenoconverted to defined neurodegenerative syndromes, in accordance with baseline biopsy results. Conclusion Even with a single 3‐mm punch biopsy, there is considerable promise for using pathological alpha‐synuclein deposition in skin to diagnose both clinical and prodromal PD. © 2020 International Parkinson and Movement Disorder Society