Chemical incorporation of 1-methyladenosine into oligonucleotides

The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.

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Bibliographic Details
Published in:Nucleic acids research 2002-03, Vol.30 (5), p.1124-1131
Main Authors: Mikhailov, Sergey N, Rozenski, Jef, Efimtseva, Ekaterina V, Busson, Roger, Van Aerschot, Arthur, Herdewijn, Piet
Format: Article
Language:English
Subjects:
1-N-methyladenosine
Adenosine - analogs & derivatives
Adenosine - chemistry
Hot Temperature
Mass Spectrometry
Nuclear Magnetic Resonance, Biomolecular
Nucleic Acid Denaturation
nucleosides
Oligodeoxyribonucleotides - chemical synthesis
Oligoribonucleotides - chemical synthesis
Organophosphorus Compounds - chemical synthesis
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Description
Summary:The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.
ISSN:1362-4962
0305-1048
1362-4962
DOI:10.1093/nar/30.5.1124