TPD1-like Gene as a Suitable Marker for Early Sex Determination in Date Palm ( Phoenix dactylifera L.)

Date palm ( L.) is a considerably beneficial and economically profitable fruit crop. Female date palm plants produce fruit that is rich in fiber and sugar. Date palm is propagated by two means: suckers and seed. The propagation of date palm through seeds is very necessary for germplasm conservation...

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Bibliographic Details
Published in:Genes 2023-04, Vol.14 (4), p.907
Main Authors: Khanum, Plosha, Khan, Asif Ali, Khan, Iqrar Ahmad, Ghaffar, Abdul, Khan, Zulqurnain
Format: Article
Language:English
Subjects:
Breeding
Crops
Cultivars
Fruit
Gene expression
Genotypes
Germplasm
Kinases
Leaves
Morphology
Phoeniceae - genetics
Phoenix dactylifera
Plant Breeding
Polymerase Chain Reaction
Propagation
Seedlings
Seedlings - genetics
Seeds
Sex determination
Sex differentiation
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Summary:Date palm ( L.) is a considerably beneficial and economically profitable fruit crop. Female date palm plants produce fruit that is rich in fiber and sugar. Date palm is propagated by two means: suckers and seed. The propagation of date palm through seeds is very necessary for germplasm conservation and breeding. The late reproductive age (4-5 years) and dioecious nature of date palm make genetic improvement and breeding difficult. Early sex determination is the only way to improve breeding by selecting experimental male and female plants at the seedling stage. The primers for ( ) were designed using Amplify software. The DNA amplification of selected date palm suckers of three genotypes (Ajwa, Amber, and Medjool) was observed through PCR. Expression profiling of selected genotypes was carried out through semi-q PCR and RT-PCR by using the cDNA of suckers and unknown seedlings. Different in silico analyses were performed for the gene and protein characterization and identification of cis-acting elements in the promoter region. The promoter was identified along with the protein's properties and functionality. The expression of gene was found in the leaves of three selected genotypes of male sucker and in some plants of selected unknown seedlings that are considered male plants, and no expression was observed in female suckers and unknown seedlings that are considered female plants. The findings suggested that the gene has the potential for sex differentiation at the seedling stage, as the gene is essential to the specialization of tapetal cells and plays a critical role in plant reproduction.
ISSN:2073-4425
2073-4425
DOI:10.3390/genes14040907