Revealing the presence of tear extracellular vesicles in Keratoconus

Extracellular vesicles (EVs) are lipid-bound vesicles that originate from the endosomal system or budded off from the plasma membrane. EVs are involved in cell-cell communication via transporting DNA, RNA, and proteins from one cell to another. Tear EVs (tEVs) have been reported in dry eye, Sjӧgren&...

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Bibliographic Details
Published in:Experimental eye research 2022-11, Vol.224, p.109242-109242, Article 109242
Main Authors: Hefley, Brenna S., Deighan, Clayton, Vasini, Brenda, Khan, Asher, Hjortdal, Jesper, Riaz, Kamran M., Liu, Yutao, Karamichos, Dimitrios
Format: Article
Language:English
Subjects:
Cornea
Extracellular vesicles
Extracellular Vesicles - metabolism
Female
Glaucoma, Open-Angle - metabolism
Humans
Keratoconus
Keratoconus - metabolism
Lipids
Male
Phenotype
RNA - genetics
RNA - metabolism
Tear
Tears - metabolism
Tetraspanin
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Summary:Extracellular vesicles (EVs) are lipid-bound vesicles that originate from the endosomal system or budded off from the plasma membrane. EVs are involved in cell-cell communication via transporting DNA, RNA, and proteins from one cell to another. Tear EVs (tEVs) have been reported in dry eye, Sjӧgren's Syndrome, and primary open-angle glaucoma. In this study, we sought to investigate the presence of tEVs in relation to keratoconus (KC). Tears were passively collected from the lateral meniscus from 10 healthy (5 males and 5 females) and 9 KC (4 males and 5 females) subjects. Tear samples were processed and analyzed using the ExoView™ R100. Statistical analysis was performed using a Mann-Whitney U non-parametric Student's t-test. All tEVs, in both Healthy and KC subjects, showed a CD9+ dominant tEV cohort independent of sex. A significant decrease in CD63+/CD9+ and CD63+/CD81+/CD9+ was found in the male KC tEVs (p 
ISSN:0014-4835
1096-0007
DOI:10.1016/j.exer.2022.109242