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Pharmacological Prospects of Morin Conjugated Selenium Nanoparticles—Evaluation of Antimicrobial, Antioxidant, Thrombolytic, and Anticancer Activities
Selenium nanoparticles (SeNPs) have gained wide importance in the scientific community and have emerged as an optimistic therapeutic carrier agent for targeted drug delivery. In the present study, the effectiveness of nano selenium conjugated with Morin (Ba-SeNp-Mo) produced from endophytic bacteria...
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| Published in: | BioNanoScience 2023-12, Vol.13 (4), p.2469-2482 |
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| creator | Nirmala, C. Sridevi, M. Aishwarya, A. Perara, Richard Sathiyanarayanan, Y. |
| description | Selenium nanoparticles (SeNPs) have gained wide importance in the scientific community and have emerged as an optimistic therapeutic carrier agent for targeted drug delivery. In the present study, the effectiveness of nano selenium conjugated with Morin (Ba-SeNp-Mo) produced from endophytic bacteria
Bacillus endophyticus
reported in our earlier research was tested against various Gram-positive, Gram-negative bacterial pathogens and fungal pathogens that showed good zone of inhibition against all selected pathogens. Antioxidant activities of these NPs were studied by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2,2′-Azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS), hydrogen peroxide (H
2
O
2
), superoxide (O
2
−
), and nitric oxide (NO) radical scavenging assays that exhibited dose-dependent free radical scavenging activity with IC
50
values 6.92 ± 1.0, 16.85 ± 1.39, 31.60 ± 1.36, 18.87 ± 1.46, and 6.95 ± 1.27 μg/mL. The efficiency of DNA cleavage and thrombolytic activity of Ba-SeNp-Mo were also studied. The antiproliferative effect of Ba-SeNp-Mo was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in COLON-26 cell lines that resulted in IC
50
value of 63.11 μg/mL. Further increased intracellular reactive oxygen species (ROS) levels up to 2.03 and significant early, late and necrotic cells were also observed in AO/EtBr assay. CASPASE 3 expression was upregulated to 1.22 (40 μg/mL) and 1.85 (80 μg/mL) fold. Thus, the current investigation suggested that the Ba-SeNp-Mo has offered remarkable pharmacological activity.
Graphical Abstract |
| doi_str_mv | 10.1007/s12668-023-01116-y |
| format | article |
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Bacillus endophyticus
reported in our earlier research was tested against various Gram-positive, Gram-negative bacterial pathogens and fungal pathogens that showed good zone of inhibition against all selected pathogens. Antioxidant activities of these NPs were studied by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2,2′-Azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS), hydrogen peroxide (H
2
O
2
), superoxide (O
2
−
), and nitric oxide (NO) radical scavenging assays that exhibited dose-dependent free radical scavenging activity with IC
50
values 6.92 ± 1.0, 16.85 ± 1.39, 31.60 ± 1.36, 18.87 ± 1.46, and 6.95 ± 1.27 μg/mL. The efficiency of DNA cleavage and thrombolytic activity of Ba-SeNp-Mo were also studied. The antiproliferative effect of Ba-SeNp-Mo was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in COLON-26 cell lines that resulted in IC
50
value of 63.11 μg/mL. Further increased intracellular reactive oxygen species (ROS) levels up to 2.03 and significant early, late and necrotic cells were also observed in AO/EtBr assay. CASPASE 3 expression was upregulated to 1.22 (40 μg/mL) and 1.85 (80 μg/mL) fold. Thus, the current investigation suggested that the Ba-SeNp-Mo has offered remarkable pharmacological activity.
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Bacillus endophyticus
reported in our earlier research was tested against various Gram-positive, Gram-negative bacterial pathogens and fungal pathogens that showed good zone of inhibition against all selected pathogens. Antioxidant activities of these NPs were studied by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2,2′-Azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS), hydrogen peroxide (H
2
O
2
), superoxide (O
2
−
), and nitric oxide (NO) radical scavenging assays that exhibited dose-dependent free radical scavenging activity with IC
50
values 6.92 ± 1.0, 16.85 ± 1.39, 31.60 ± 1.36, 18.87 ± 1.46, and 6.95 ± 1.27 μg/mL. The efficiency of DNA cleavage and thrombolytic activity of Ba-SeNp-Mo were also studied. The antiproliferative effect of Ba-SeNp-Mo was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in COLON-26 cell lines that resulted in IC
50
value of 63.11 μg/mL. Further increased intracellular reactive oxygen species (ROS) levels up to 2.03 and significant early, late and necrotic cells were also observed in AO/EtBr assay. CASPASE 3 expression was upregulated to 1.22 (40 μg/mL) and 1.85 (80 μg/mL) fold. Thus, the current investigation suggested that the Ba-SeNp-Mo has offered remarkable pharmacological activity.
Graphical Abstract</description><subject>Anticancer properties</subject><subject>Antioxidants</subject><subject>Assaying</subject><subject>Bacteria</subject><subject>Biological and Medical Physics</subject><subject>Biomaterials</subject><subject>Biophysics</subject><subject>Caspase-3</subject><subject>Cell lines</subject><subject>Circuits and Systems</subject><subject>Drug delivery</subject><subject>Endophytes</subject><subject>Engineering</subject><subject>Free radicals</subject><subject>Hydrogen peroxide</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nitric oxide</subject><subject>Pathogens</subject><subject>Pharmacology</subject><subject>Reactive oxygen species</subject><subject>Scavenging</subject><subject>Selenium</subject><subject>Sulfonic acid</subject><subject>Thrombolysis</subject><issn>2191-1630</issn><issn>2191-1649</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kctuEzEUhkcIRKvSF2CBRmLDIgM-9ownXqEqKhepQCXK2jrj8SSOPHawPRHZ8RAseD6eBDcp4bLAG1_O9__28V8Uj4E8B0LaFxEo5_OKUFYRAODV7l5xSkFABbwW949rRk6K8xjXJI-WcDZnD4sT1jIOQOhp8f16hWFE5a1fGoW2vA4-brRKsfRD-c4H48qFd-tpiUn35UdttTPTWL5H5zcYklFWxx9fv11u0U6YjHe3uguXzGhU8J1BO9tv_RfTo0uz8mYV_Nh5u8vaWYmu35cVOqVDeaGS2ZpkdHxUPBjQRn1-N58Vn15d3izeVFcfXr9dXFxVqm6bVKm2VRxr1Q951VNG55RrUB2QRomBoNKD0IQTnc_rVvQKETvRzDuAthacsLPi5cF3M3Wj7pV2KaCVm2BGDDvp0ci_K86s5NJvJRDgAijNDs_uHIL_POmY5Gii0tai036Kks4ZodAANBl9-g-69lNwub9MCSbqRtA2U_RA5Q-MMejh-Bog8jZ8eQhf5vDlPny5y6Inf_ZxlPyKOgPsAMRccksdft_9H9uf1Py_3A</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Nirmala, C.</creator><creator>Sridevi, M.</creator><creator>Aishwarya, A.</creator><creator>Perara, Richard</creator><creator>Sathiyanarayanan, Y.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-5975-9244</orcidid><orcidid>https://orcid.org/0000-0002-4039-3393</orcidid></search><sort><creationdate>20231201</creationdate><title>Pharmacological Prospects of Morin Conjugated Selenium Nanoparticles—Evaluation of Antimicrobial, Antioxidant, Thrombolytic, and Anticancer Activities</title><author>Nirmala, C. ; Sridevi, M. ; Aishwarya, A. ; Perara, Richard ; Sathiyanarayanan, Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-c77c6a4cdfc77d232826e1cb105c9f0acef9e060e826479dcaaab958b11749603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anticancer properties</topic><topic>Antioxidants</topic><topic>Assaying</topic><topic>Bacteria</topic><topic>Biological and Medical Physics</topic><topic>Biomaterials</topic><topic>Biophysics</topic><topic>Caspase-3</topic><topic>Cell lines</topic><topic>Circuits and Systems</topic><topic>Drug delivery</topic><topic>Endophytes</topic><topic>Engineering</topic><topic>Free radicals</topic><topic>Hydrogen peroxide</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nitric oxide</topic><topic>Pathogens</topic><topic>Pharmacology</topic><topic>Reactive oxygen species</topic><topic>Scavenging</topic><topic>Selenium</topic><topic>Sulfonic acid</topic><topic>Thrombolysis</topic><toplevel>online_resources</toplevel><creatorcontrib>Nirmala, C.</creatorcontrib><creatorcontrib>Sridevi, M.</creatorcontrib><creatorcontrib>Aishwarya, A.</creatorcontrib><creatorcontrib>Perara, Richard</creatorcontrib><creatorcontrib>Sathiyanarayanan, Y.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioNanoScience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nirmala, C.</au><au>Sridevi, M.</au><au>Aishwarya, A.</au><au>Perara, Richard</au><au>Sathiyanarayanan, Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pharmacological Prospects of Morin Conjugated Selenium Nanoparticles—Evaluation of Antimicrobial, Antioxidant, Thrombolytic, and Anticancer Activities</atitle><jtitle>BioNanoScience</jtitle><stitle>BioNanoSci</stitle><addtitle>Bionanoscience</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>13</volume><issue>4</issue><spage>2469</spage><epage>2482</epage><pages>2469-2482</pages><issn>2191-1630</issn><eissn>2191-1649</eissn><abstract>Selenium nanoparticles (SeNPs) have gained wide importance in the scientific community and have emerged as an optimistic therapeutic carrier agent for targeted drug delivery. In the present study, the effectiveness of nano selenium conjugated with Morin (Ba-SeNp-Mo) produced from endophytic bacteria
Bacillus endophyticus
reported in our earlier research was tested against various Gram-positive, Gram-negative bacterial pathogens and fungal pathogens that showed good zone of inhibition against all selected pathogens. Antioxidant activities of these NPs were studied by 1, 1-diphenyl-2- picrylhydrazyl (DPPH), 2,2′-Azino-bis-3-ethylbenzothiozoline-6-sulfonic acid (ABTS), hydrogen peroxide (H
2
O
2
), superoxide (O
2
−
), and nitric oxide (NO) radical scavenging assays that exhibited dose-dependent free radical scavenging activity with IC
50
values 6.92 ± 1.0, 16.85 ± 1.39, 31.60 ± 1.36, 18.87 ± 1.46, and 6.95 ± 1.27 μg/mL. The efficiency of DNA cleavage and thrombolytic activity of Ba-SeNp-Mo were also studied. The antiproliferative effect of Ba-SeNp-Mo was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in COLON-26 cell lines that resulted in IC
50
value of 63.11 μg/mL. Further increased intracellular reactive oxygen species (ROS) levels up to 2.03 and significant early, late and necrotic cells were also observed in AO/EtBr assay. CASPASE 3 expression was upregulated to 1.22 (40 μg/mL) and 1.85 (80 μg/mL) fold. Thus, the current investigation suggested that the Ba-SeNp-Mo has offered remarkable pharmacological activity.
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| subjects | Anticancer properties Antioxidants Assaying Bacteria Biological and Medical Physics Biomaterials Biophysics Caspase-3 Cell lines Circuits and Systems Drug delivery Endophytes Engineering Free radicals Hydrogen peroxide Nanoparticles Nanotechnology Nitric oxide Pathogens Pharmacology Reactive oxygen species Scavenging Selenium Sulfonic acid Thrombolysis |
| title | Pharmacological Prospects of Morin Conjugated Selenium Nanoparticles—Evaluation of Antimicrobial, Antioxidant, Thrombolytic, and Anticancer Activities |
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