Enhancement of clearance of bacteria from murine lungs by immunization with detoxified lipooligosaccharide from Moraxella catarrhalis conjugated to proteins

Moraxella catarrhalis strain 25238 detoxified lipooligosaccharide (dLOS)-protein conjugates induced a significant rise of bactericidal anti-LOS antibodies in animals. This study reports the effect of active or passive immunization with the conjugates or their antiserum on pulmonary clearance of M. c...

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Bibliographic Details
Published in:Infection and immunity 2000-09, Vol.68 (9), p.4980-4985
Main Authors: HU, W.-G, JING CHEN, BATTEY, J. F, GU, X.-X
Format: Article
Language:English
Subjects:
Animals
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Female
Fundamental and applied biological sciences. Psychology
Haemophilus influenzae
Immunization
Immunization, Passive
Lipooligosaccharides
Lipopolysaccharides - immunology
Lung - immunology
Lung - microbiology
Lung - pathology
Mice
Mice, Inbred BALB C
Microbial Immunity and Vaccines
Microbiology
Moraxella (Branhamella) catarrhalis - immunology
Moraxella catarrhalis
Rabbits
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Moraxella catarrhalis strain 25238 detoxified lipooligosaccharide (dLOS)-protein conjugates induced a significant rise of bactericidal anti-LOS antibodies in animals. This study reports the effect of active or passive immunization with the conjugates or their antiserum on pulmonary clearance of M. catarrhalis in an aerosol challenge mouse model. Mice were injected subcutaneously with dLOS-tetanus toxoid (dLOS-TT), dLOS-high-molecular-weight proteins (dLOS-HMP) from nontypeable Haemophilus influenzae (NTHi), or nonconjugated materials in Ribi adjuvant and then challenged with M. catarrhalis strain 25238 or O35E or NTHi strain 12. Immunization with dLOS-TT or dLOS-HMP generated a significant rise of serum anti-LOS immunoglobulin G and 68% and 35 to 41% reductions of bacteria in lungs compared with the control (P
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.68.9.4980-4985.2000