Loading…

Boholamide A, an APD-Class, Hypoxia-Selective Cyclodepsipeptide

Calcium homeostasis is implicated in some cancers, leading to the possibility that selective control of calcium might lead to new cancer drugs. On the basis of this idea, we designed an assay using a glioblastoma cell line and screened a collection of 1000 unique bacterial extracts. Isolation of the...

Full description

Saved in:
Bibliographic Details
Published in:Journal of natural products (Washington, D.C.) D.C.), 2020-04, Vol.83 (4), p.1249-1257
Main Authors: Torres, Joshua P, Lin, Zhenjian, Fenton, David S, Leavitt, Lee U, Niu, Changshan, Lam, Pui-Ying, Robes, Jose Miguel, Peterson, Randall T, Concepcion, Gisela P, Haygood, Margo G, Olivera, Baldomero M, Schmidt, Eric W
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Calcium homeostasis is implicated in some cancers, leading to the possibility that selective control of calcium might lead to new cancer drugs. On the basis of this idea, we designed an assay using a glioblastoma cell line and screened a collection of 1000 unique bacterial extracts. Isolation of the active compound from a hit extract led to the identification of boholamide A (1), a 4-amido-2,4-pentadieneoate (APD)-class peptide. Boholamide A (1) applied in the nanomolar range induces an immediate influx of Ca2+ in glioblastoma and neuronal cells. APD-class natural products are hypoxia-selective cytotoxins that primarily target mitochondria. Like other APD-containing compounds, 1 is hypoxia selective. Since APD natural products have received significant interest as potential chemotherapeutic agents, 1 provides a novel APD scaffold for the development of new anticancer compounds.
ISSN:0163-3864
1520-6025
DOI:10.1021/acs.jnatprod.0c00038