Molecular detection of Wolbachia and Bartonella as part of the microbiome of phlebotomine sand flies from Chiapas, Mexico

Phlebotomine sand flies are dipterans of relevance due to their role as vectors of several pathogens worldwide. Bacteria in the gut of sand flies possibly affect their vectorial capacity and competence to transmit parasites. A retrospective study was performed in sand fly specimens that had previous...

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Bibliographic Details
Published in:Parasitology research (1987) 2023-06, Vol.122 (6), p.1293-1301
Main Authors: Lozano-Sardaneta, Yokomi N., Marina, Carlos F., Torres-Monzón, Jorge A., Sánchez-Cordero, Víctor, Becker, Ingeborg
Format: Article
Language:English
Subjects:
Analysis
Animals
Bacteria
Bartonella
Bartonella - genetics
Biomedical and Life Sciences
Biomedicine
Disease transmission
Immunology
Leishmania
Meal feeding
Medical Microbiology
Mexico
Microbiology
Microbiomes
Microbiota
Phlebotomus
Phylogeny
Protozoa
Psychodidae - parasitology
Retrospective Studies
Strains (organisms)
Vectors
Wolbachia
Wolbachia - genetics
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Summary:Phlebotomine sand flies are dipterans of relevance due to their role as vectors of several pathogens worldwide. Bacteria in the gut of sand flies possibly affect their vectorial capacity and competence to transmit parasites. A retrospective study was performed in sand fly specimens that had previously been collected in four localities of the state of Chiapas during the period 2009–2011 to detect Wolbachia and Bartonella and their possible coinfection with Leishmania . For the molecular detection of bacteria, we used primers and conditions that had previously been reported. A total of 531 sand fly specimens of 10 species were analyzed. Four Wolbachia strains were detected in five sand fly species, showing a prevalence of 8.6%. All the Wolbachia strains had previously been reported in other taxa. In one sand fly species, we also detected a new lineage of Bartonella evidenced by a phylogenetic analysis. No sand fly specimens showed coinfections of these bacteria and Leishmania . The bacteria found in the phlebotomine sand flies are possibly transmitted by plant-mediated horizontal transmission and during blood meal feeding.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-023-07829-z