Alterations in the root phenylpropanoid pathway and root–shoot vessel system as main determinants of the drought tolerance of a soybean genotype

Climate change increases precipitation variability, particularly in savanna environments. We have used integrative strategies to understand the molecular mechanisms of drought tolerance, which will be crucial for developing improved genotypes. The current study compares the molecular and physiologic...

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Published in:Physiology and molecular biology of plants 2023-04, Vol.29 (4), p.559-577
Main Authors: Coutinho, Flaviane Silva, Mesquita, Rosilene Oliveira, Rodrigues, Juliano Mendonça, Zanotti, Analú, Faustino, Verônica Aparecida, Barros, Edvaldo, Vital, Camilo Elber, de Almeida Oliveira, Maria Goreti, Meira, Renata Maria Strozi Alves, Williams, Thomas Christopher Rhys, Fontes, Elizabeth Pacheco Batista, Loureiro, Marcelo Ehlers, de Oliveira Ramos, Humberto Josué
Format: Article
Language:English
Subjects:
Abscisic acid
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biosynthesis
Cell Biology
Cell walls
Climate change
Drought
Drought resistance
Genotypes
Glutamine
Life Sciences
Molecular modelling
Oxidative stress
Parameter sensitivity
Phenols
Physiology
Plant growth
Plant Physiology
Plant Sciences
Proteins
Proteolysis
Proteomes
Proteomics
Research Article
Shoots
Soybeans
Substrates
Transcriptomes
Vessels
Water absorption
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Summary:Climate change increases precipitation variability, particularly in savanna environments. We have used integrative strategies to understand the molecular mechanisms of drought tolerance, which will be crucial for developing improved genotypes. The current study compares the molecular and physiological parameters between the drought-tolerant Embrapa 48 and the sensitive BR16 genotypes. We integrated the root–shoot system’s transcriptome, proteome, and metabolome to understand drought tolerance. The results indicated that Embrapa 48 had a greater capacity for water absorption due to alterations in length and volume. Drought tolerance appears to be ABA-independent, and IAA levels in the leaves partially explain the higher root growth. Proteomic profiles revealed up-regulated proteins involved in glutamine biosynthesis and proteolysis, suggesting osmoprotection and explaining the larger root volume. Dysregulated proteins in the roots belong to the phenylpropanoid pathways. Additionally, PR-like proteins involved in the biosynthesis of phenolics may act to prevent oxidative stress and as a substrate for modifying cell walls. Thus, we concluded that alterations in the root–shoot conductive vessel system are critical in promoting drought tolerance. Moreover, photosynthetic parameters from reciprocal grafting experiments indicated that the root system is more essential than the shoots in the drought tolerance mechanism. Finally, we provided a comprehensive overview of the genetic, molecular, and physiological traits involved in drought tolerance mechanisms.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-023-01307-7