Anticancer Potential of Sulfonamide Moieties via In-Vitro and In-Silico Approaches: Comparative Investigations for Future Drug Development

• Published in: International journal of molecular sciences 2023-04, Vol.24 (9), p.7953
• Main Authors: Wani, Tanveer A, Zargar, Seema, Alkahtani, Hamad M, Altwaijry, Nojood, Al-Rasheed, Lamees S
• Format: Article
• Language: English
• Subjects: Antibiotics; Antineoplastic Agents - chemistry; Apoptosis; Binding; Bioactive compounds; Biocompatibility; Cancer therapies; Cell cycle; Cell growth; Cell Line, Tumor; Cell Proliferation; Cervical cancer; Cisplatin; Cisplatin - pharmacology; Comparative analysis; Computer applications; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA - metabolism; Doxorubicin; Doxorubicin - pharmacology; Drug Development; Drug interaction; Drug resistance; Drug Screening Assays, Antitumor; Drugs; Energy; Fatalities; Free energy; Grooves; HeLa Cells; Humans; Investigations; Ligands; Male; Molecular docking; Molecular Docking Simulation; Molecular dynamics; Molecular Structure; Mortality; Optimization; Pharmaceutical sciences; Proteins; Semen - metabolism; Structure-Activity Relationship; Sulfamide; Sulfonamides; Sulfonamides - pharmacology; Toluene
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24097953

Several kinds of anticancer drugs are presently commercially accessible, but low efficacy, solubility, and toxicity have reduced the overall therapeutic indices. Thus, the search for promising anticancer drugs continues. The interactions of numerous essential anticancer drugs with DNA are crucial to their biological functions. Here, the anticancer effects of N-ethyl toluene-4-sulphonamide ( ) and 2,5-Dichlorothiophene-3-sulphonamide ( ) on cell lines from breast and cervical cancer were investigated. The study also compared how these substances interacted with the hearing sperm DNA. The most promising anticancer drug was identified as 2,5-Dichlorothiophene-3-sulfonamide ( ), which showed GI of 7.2 ± 1.12 µM, 4.62 ± 0.13 µM and 7.13 ± 0.13 µM against HeLa, MDA-MB231 and MCF-7 cells, respectively. Moreover, it also exhibited significant electrostatic and non-electrostatic contributions to the binding free energy. The work utilized computational techniques, such as molecular docking and molecular dynamic (MD) simulations, to demonstrate the strong cytotoxicity of 2,5-Dichlorothiophene-3-sulfamide ( ) in comparison to standard Doxorubicin and cisplatin, respectively. Molecular docking experiments provided additional support for a role for the minor groove in the binding of the 2,5-Dichlorothiophene-3-sulfamide ( )-DNA complex. The molecular docking studies and MD simulation showed that both compounds revealed comparable inhibitory potential against standard Doxorubicin and cisplatin. This study has the potential to lead to the discovery of new bioactive compounds for use in cancer treatment, including metallic and non-metallic derivatives of 2,5-Dichlorothiophene-3-sulfonamide ( ). It also emphasizes the worth of computational approaches in the development of new drugs and lays the groundwork for future research.