Plum-Derived Exosome-like Nanovesicles Induce Differentiation of Osteoblasts and Reduction of Osteoclast Activation

Osteoblasts and osteoclasts play crucial roles in bone formation and bone resorption. We found that plum-derived exosome-like nanovesicles (PENVs) suppressed osteoclast activation and modulated osteoblast differentiation. PENVs increased the proliferation, differentiation, and mineralization of oste...

Full description

Saved in:
Bibliographic Details
Published in:Nutrients 2023-04, Vol.15 (9), p.2107
Main Authors: Park, Yu-Seong, Kim, Hyun-Woo, Hwang, Jin-Hyeon, Eom, Jung-Young, Kim, Dong-Ha, Park, Jinho, Tae, Hyun-Jin, Lee, Seunghoon, Yoo, Jae-Gyu, Kim, Jee-In, Lim, Jae-Hwan, Kwun, In-Sook, Baek, Moon-Chang, Cho, Young-Eun, Kim, Do-Kyun
Format: Article
Language:English
Subjects:
Acids
Arthritis
Bone growth
Bone marrow
Bone morphogenetic protein 2
Bone resorption
c-Fos protein
Cell culture
Cell differentiation
Cell proliferation
Differentiation
DNA binding proteins
Enzymes
Ethylenediaminetetraacetic acid
Microscopy
Mineralization
Osteoblastogenesis
Osteoblasts
Osteoclastogenesis
Osteoclasts
Osteogenesis
Penicillin
Phosphatase
Polyphenols
Proteins
Reagents
Stem cells
Therapeutic targets
Transcription factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Osteoblasts and osteoclasts play crucial roles in bone formation and bone resorption. We found that plum-derived exosome-like nanovesicles (PENVs) suppressed osteoclast activation and modulated osteoblast differentiation. PENVs increased the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells and osteoblasts from mouse bone marrow cultures. Notably, PENVs elevated the expression of osteoblastic transcription factors and osteoblast differentiation marker proteins in MC3T3-E1 cells. Higher levels of phosphorylated BMP-2, p38, JNK, and smad1 proteins were detected in PENV-treated MC3T3-E1 cells. Additionally, the number of TRAP-positive cells was significantly decreased in PENV-treated osteoclasts isolated from osteoblasts from mouse bone marrow cultures. Importantly, osteoclastogenesis of marker proteins such as PPAR-gamma, NFATc1, and c-Fos were suppressed by treatment with PENVs (50 μg/mL). Taken together, these results demonstrate that PENVs can be used as therapeutic targets for treating bone-related diseases by improving osteoblast differentiation and inhibiting osteoclast activation for the first time.
ISSN:2072-6643
2072-6643
DOI:10.3390/nu15092107